Isolation of peptides useful for differential diagnosis of Crohn's disease and ulcerative colitis.

Abstract

BACKGROUND:

Phage displayed random peptide technology has been utilised to identify binding epitopes of antibodies or receptor ligands.

AIM:

To isolates peptides from a phage library which are specifically recognised by antibodies in serum from patients with Crohn's disease (CD).

METHODS:

A phage displayed random peptide library composed of nine amino acids was established and sequentially screened using serum immunogloblin G obtained from CD patients.

RESULTS:

Five different CD specific peptides were isolated from the phage library. No homology in amino acid sequences was observed among four (CDP-1, -3 to -5) of the five peptides exhibiting different binding characteristics with each CD patient's serum. In contrast, two peptides (CDP-1 and -2) had similar amino acid sequences and similar binding characteristics. Four multiple antigenic peptides (MAP, CDP-1, -3 to -5) were synthesised, and an enzyme linked immunosorbent assay (ELISA) using the four peptides was developed to detect serum antibodies against them. Fifty two of 92 CD patients (56.5%) were detected by ELISA, none of 20 ulcerative colitis (UC) patients, only one of 25 duodenal ulcer patients, and only three of 48 healthy subjects.

CONCLUSIONS:

ELISA using the four peptides isolated in this study may be useful for the differential diagnosis of CD and UC.

• Authors: H Saito, Y Fukuda, K Katsuragi, M Tanaka, M Satomi, T Shimoyama, T Saito, T Tachikawa
• Journal: Gut (Gut) Vol. 52 Issue 4 Pg. 535-40 (Apr 2003) ISSN: 0017-5749 [Print] England
• PMID: 12631665 (Publication Type: Journal Article)

Chemical References

• Autoantibodies
• Biomarkers
• Immunoglobulin G
• Peptide Library
• Peptides

Topics

• Amino Acid Sequence
• Autoantibodies (blood, immunology)
• Biomarkers (blood)
• Colitis, Ulcerative (diagnosis, immunology)
• Crohn Disease (diagnosis, immunology)
• Diagnosis, Differential
• Enzyme-Linked Immunosorbent Assay (methods)
• Humans
• Immunoglobulin G (immunology)
• Molecular Sequence Data
• Peptide Library
• Peptides (immunology)
• ROC Curve
• Serologic Tests (methods)