Epithelial neutrophil-activating peptide 78 concentrations are elevated in the peritoneal fluid of women with endometriosis.

Abstract

OBJECTIVE:

To investigate the presence of epithelial neutrophil-activating peptide 78 (ENA-78) in peritoneal fluid of women with and without endometriosis and to identify the cells that produce this inflammatory protein.

DESIGN:

Case-control study.

SETTING:

University hospital.

PATIENT(S):

Eighteen women with and 9 women without endometriosis.

MAIN OUTCOME MEASURE(S):

ENA-78 protein and mRNA levels were compared among women with and without endometriosis in samples of peritoneal fluid, samples of endometriotic lesions obtained by biopsy during laparoscopy, and peritoneal macrophages. Enzyme-linked immunosorbent assay, reverse transcription polymerase chain reaction, and in situ hybridization methods were used. Secretion of ENA-78 protein by interleukin-1beta-stimulated endometriotic stromal cells and in the media of lipopolysaccharide-stimulated peritoneal macrophages were compared to that in unstimulated cell cultures.

RESULT(S):

Peritoneal fluid concentrations of ENA-78 were significantly higher in affected women than in controls. Ectopic epithelial and stromal cells and peritoneal macrophages express ENA-78 messenger RNA. Interleukin-1beta stimulation of stromal cell cultures resulted in a 23-fold increase in ENA-78 concentration, and lipopolysaccharide stimulation of peritoneal macrophages increased concentrations by 8-fold.

CONCLUSION(S):

Levels of ENA-78 are elevated in the peritoneal fluid of women with endometriosis. Ectopic glandular cells, ectopic stromal cells, and peritoneal macrophages express this inflammatory chemokine. Epithelial neutrophil-activating peptide 78 may play an important role in the pathogenesis of endometriosis.

• Authors: Michael D Mueller, Luca Mazzucchelli, Caroline Buri, Dan I Lebovic, Ekkehard Dreher, Robert N Taylor
• Journal: Fertility and sterility (Fertil Steril) Vol. 79 Suppl 1 Pg. 815-20 (Mar 2003) ISSN: 0015-0282 [Print] United States
• PMID: 12620496 (Publication Type: Journal Article)

Chemical References

• CXCL5 protein, human
• Chemokine CXCL5
• Chemokines, CXC
• Interleukin-1
• Interleukin-8
• Lipopolysaccharides
• RNA, Messenger

Topics

• Ascitic Fluid (metabolism)
• Case-Control Studies
• Chemokine CXCL5
• Chemokines, CXC
• Endometriosis (metabolism)
• Female
• Humans
• In Situ Hybridization
• Interleukin-1 (pharmacology)
• Interleukin-8 (analogs & derivatives, biosynthesis, genetics)
• Lipopolysaccharides (pharmacology)
• Macrophages, Peritoneal (immunology, metabolism)
• RNA, Messenger (biosynthesis, genetics)
• Reverse Transcriptase Polymerase Chain Reaction