Characterisation of the 1H and 13C NMR spectra of N-acetylaspartylglutamate and its detection in urine from patients with Canavan disease.

Abstract

1H and 13C NMR spectra of N-acetylaspartylglutamate (NAAG) have been recorded and interpreted. The values of the 1H chemical shifts and 1H-(1)H coupling constants at different pH were obtained by iterative computer fitting of 1-D 1H NMR spectra. This provided information on the solution conformation of the investigated molecule. Proton-decoupled high resolution 13C NMR spectra of NAAG have been measured in a series of dilute water solution of various acidity. These data have provided a basis for unequivocal determination of the presence of NAAG in the urine sample of a patient suffering from Canavan disease. NMR spectroscopy provides a possibility of detecting NAAG in body fluids.

• Authors: Hanna Krawczyk, Wanda Gradowska
• Journal: Journal of pharmaceutical and biomedical analysis (J Pharm Biomed Anal) Vol. 31 Issue 3 Pg. 455-63 (Mar 10 2003) ISSN: 0731-7085 [Print] England
• PMID: 12615232 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Dipeptides
• Indicators and Reagents
• Protons
• isospaglumic acid

Topics

• Canavan Disease (urine)
• Child
• Dipeptides (analysis, urine)
• Humans
• Hydrogen-Ion Concentration
• Indicators and Reagents
• Magnetic Resonance Spectroscopy
• Protons