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Biological and environmental factors affecting ultrasound-induced hemolysis in vitro: 3. Antioxidant (Trolox) inclusion.

Abstract
This project tested the hypothesis that human erythrocytes pretreated with Trolox (a water-soluble analog of vitamin E) would be more susceptible to ultrasound (US)-induced hemolysis by a cavitational mechanism because of an increased fragility of the erythrocyte membrane over that without Trolox supplementation. Samples of whole human blood from apparently healthy donors (hematocrit approximately 40%) in vitro were supplemented or not supplemented with Trolox at various concentrations, ranging from 1.8 to 0.0018 mg/mL plasma. Mechanical fragility tests indicated the Trolox-treated blood in vitro exhibited greater hemolysis than untreated blood in vitro (p < 0.001). US exposures at comparable acoustic amplitude, pulse length and duty factor in the presence of the US contrast agent Albunex yielded differing results; at 1 MHz, the Trolox-supplemented blood had significantly greater hemolysis in vitro than non-Trolox-supplemented blood; at 3 MHz, there was a substantial reduction in hemolysis relative to that obtained at 1 MHz, and no statistically significant difference between the Trolox-supplemented and -unsupplemented blood. There was also essentially no support for an alternative hypothesis that the Trolox was functioning primarily as a pro-oxidant. These collective experimental results support the hypothesis and suggest duality in the functionality of membranous antioxidant inclusions or associations; they may foster protection against oxidative damage, yet render the cell less capable of withstanding mechanical stress.
AuthorsMorton W Miller, W Marcus Miller, Linda F Battaglia
JournalUltrasound in medicine & biology (Ultrasound Med Biol) Vol. 29 Issue 1 Pg. 103-12 (Jan 2003) ISSN: 0301-5629 [Print] England
PMID12604121 (Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, P.H.S.)
CopyrightCopyright 2003 World Federation for Ultrasound in Medicine & Biology
Chemical References
  • Antioxidants
  • Chromans
  • 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid
Topics
  • Antioxidants (pharmacology)
  • Chromans (pharmacology)
  • Erythrocyte Membrane (physiology)
  • Erythrocytes (diagnostic imaging)
  • Hemolysis (drug effects)
  • Humans
  • In Vitro Techniques
  • Osmotic Fragility
  • Ultrasonography

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