Granulocyte colony-stimulating factor for the treatment of biomaterial-associated staphylococcal infections in-vitro.

Abstract

Staphylococcal infections are a common and severe complication after the implantation of a prosthesis. We developed an in-vitro model for biomaterial-associated infections and studied the effects of human recombinant granulocyte colony-stimulating factor (rhuG-CSF; filgrastime) on the eradication of bacteria from the surface of biomaterial. Latex beads (25 micro m) were incubated with 10(7) colony forming units of either a slime producing (DSM 3269) or non-slime producing strain (ATCC 14990) of Staphylococcus epidermidis. Infected particles were consecutively confronted with effector cells, derived from heparinized whole blood samples taken from healthy volunteers, after stimulation with rhuG-CSF (5,000 IU/ml, 10,000 IU/ml). Control blood specimens were not stimulated or conditioned with normal saline. The results indicate that stimulation with rhuG-CSF induced an increased rate of phagocytosis and lead to a more rapid reduction of adhering bacteria from the surface of the beads. Therefore, the in-vitro data suggest that patients with prosthesis infection may profit from an additional treatment with rhuG-CSF.

• Authors: Andrea Schlöbe, Norbert Schnitzler, Klaus Schweizer, Detlef Rohde
• Journal: Urological research (Urol Res) Vol. 30 Issue 6 Pg. 394-8 (Feb 2003) ISSN: 0300-5623 [Print] Germany
• PMID: 12599021 (Publication Type: Journal Article)

Chemical References

• Recombinant Proteins
• Granulocyte Colony-Stimulating Factor

Topics

• Adult
• Granulocyte Colony-Stimulating Factor (pharmacology)
• Humans
• In Vitro Techniques
• Microspheres
• Neutrophil Activation (drug effects)
• Neutrophils (immunology, microbiology)
• Phagocytosis (drug effects)
• Recombinant Proteins (pharmacology)
• Staphylococcal Infections (drug therapy, immunology)
• Staphylococcus epidermidis