Nuclear localization of phosphorylated c-Myc protein in human tumor cells.

Abstract

Using immunocytochemical techniques at light and electron microscopy, we analysed the distribution of phosphorylated c-Myc in actively proliferating human HeLa cells. The distribution pattern of c-Myc was also compared with those of other ribonucleoprotein (RNP)-containing components (PANA, hnRNP-core proteins, fibrillarin) or RNP-associated nuclear proteins (SC-35 splicing factor). Our results provide the first evidence that phosphorylated c-Myc accumulates in the nucleus of tumor cells, where it colocalizes with fibrillarin, both in the nucleolus and in extranucleolar structures.

• Authors: C Soldani, M G Bottone, M Biggiogera, C Alpini, A I Scovassi, T Martin, C Pellicciari
• Journal: European journal of histochemistry : EJH (Eur J Histochem) Vol. 46 Issue 4 Pg. 377-80 ( 2002) ISSN: 1121-760X [Print] Italy
• PMID: 12597623 (Publication Type: Letter, Research Support, Non-U.S. Gov't)

Chemical References

• Chromosomal Proteins, Non-Histone
• Heterogeneous-Nuclear Ribonucleoproteins
• Proto-Oncogene Proteins c-myc
• fibrillarin

Topics

• Cell Nucleolus (metabolism, ultrastructure)
• Cell Nucleus (chemistry, ultrastructure)
• Chromosomal Proteins, Non-Histone (metabolism)
• HeLa Cells
• Heterogeneous-Nuclear Ribonucleoproteins (metabolism)
• Humans
• Microscopy, Electron
• Microscopy, Fluorescence
• Neoplasms (metabolism, pathology)
• Phosphorylation
• Proto-Oncogene Proteins c-myc (analysis)