Abstract |
Ultrasonically induced cell damage and active oxygen generation with 4-formyloximeetylidene-3-hydroxyl-2-vinyl-deuterio-porphynyl(IX)-6-7-diaspartic acid (ATX-S10) were compared in the same in vitro insonation setup. Sarcoma 180 cells suspended in air-saturated PBS were exposed to ultrasound at 2 MHz for up to 60 s in the presence and absence of ATX-S10. The viability was determined by Trypan blue exclusion test. Ultrasonically induced active oxygen generation in the presence and absence of ATX-S10 in air-saturated aqueous solutions of 50 mM 2,2,6,6-tetramethyl-4-piperidone was detected by electron spin resonance (ESR). Significant enhancement of the rates of both ultrasonically induced cell damage and nitroxide generation was demonstrated with 40-160 microM ATX-S10. Both rates correlated very well. The enhancement of both rates with ATX-S10 was suppressed by 10 mM histidine. These results suggest that ultrasonically generated active oxygen plays a primary role in the ultrasonically induced cell damage in the presence of ATX-S10.
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Authors | Nagahiko Yumita, Isao Sakata, Susumu Nakajima, Shin-ichiro Umemura |
Journal | Biochimica et biophysica acta
(Biochim Biophys Acta)
Vol. 1620
Issue 1-3
Pg. 179-84
(Mar 17 2003)
ISSN: 0006-3002 [Print] Netherlands |
PMID | 12595087
(Publication Type: Journal Article)
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Chemical References |
- ATX-S10
- Free Radical Scavengers
- Nitrogen Oxides
- Porphyrins
- Reactive Oxygen Species
- Histidine
- nitroxyl
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Topics |
- Animals
- Electron Spin Resonance Spectroscopy
- Free Radical Scavengers
(chemistry)
- Histidine
(chemistry)
- Molecular Structure
- Nitrogen Oxides
(analysis, chemical synthesis)
- Porphyrins
(antagonists & inhibitors)
- Reactive Oxygen Species
(analysis, chemistry)
- Sarcoma 180
- Tumor Cells, Cultured
- Ultrasonics
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