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Ultrasonically induced cell damage and active oxygen generation by 4-formyloximeetylidene-3-hydroxyl-2-vinyl-deuterio-porphynyl(IX)-6-7-diaspartic acid: on the mechanism of sonodynamic activation.

Abstract
Ultrasonically induced cell damage and active oxygen generation with 4-formyloximeetylidene-3-hydroxyl-2-vinyl-deuterio-porphynyl(IX)-6-7-diaspartic acid (ATX-S10) were compared in the same in vitro insonation setup. Sarcoma 180 cells suspended in air-saturated PBS were exposed to ultrasound at 2 MHz for up to 60 s in the presence and absence of ATX-S10. The viability was determined by Trypan blue exclusion test. Ultrasonically induced active oxygen generation in the presence and absence of ATX-S10 in air-saturated aqueous solutions of 50 mM 2,2,6,6-tetramethyl-4-piperidone was detected by electron spin resonance (ESR). Significant enhancement of the rates of both ultrasonically induced cell damage and nitroxide generation was demonstrated with 40-160 microM ATX-S10. Both rates correlated very well. The enhancement of both rates with ATX-S10 was suppressed by 10 mM histidine. These results suggest that ultrasonically generated active oxygen plays a primary role in the ultrasonically induced cell damage in the presence of ATX-S10.
AuthorsNagahiko Yumita, Isao Sakata, Susumu Nakajima, Shin-ichiro Umemura
JournalBiochimica et biophysica acta (Biochim Biophys Acta) Vol. 1620 Issue 1-3 Pg. 179-84 (Mar 17 2003) ISSN: 0006-3002 [Print] Netherlands
PMID12595087 (Publication Type: Journal Article)
Chemical References
  • ATX-S10
  • Free Radical Scavengers
  • Nitrogen Oxides
  • Porphyrins
  • Reactive Oxygen Species
  • Histidine
  • nitroxyl
Topics
  • Animals
  • Electron Spin Resonance Spectroscopy
  • Free Radical Scavengers (chemistry)
  • Histidine (chemistry)
  • Molecular Structure
  • Nitrogen Oxides (analysis, chemical synthesis)
  • Porphyrins (antagonists & inhibitors)
  • Reactive Oxygen Species (analysis, chemistry)
  • Sarcoma 180
  • Tumor Cells, Cultured
  • Ultrasonics

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