Mobilization of peripheral blood progenitor cells with a combination of cyclophosphamide, r-metHuSCF and filgrastim in patients with breast cancer previously treated with chemotherapy.

Abstract

The objective of our study was to determine the effect of adding r-metHuSCF to Filgrastim and cyclophosphamide for mobilization of peripheral blood progenitor cells (PBPC), on collection of CD34(+) cells and engraftment after autologous stem cell transplant. Twenty-three patients with previously treated stage II-IV breast cancer received cyclophosphamide (3 g/m(2)), Filgrastim 5 microg/kg daily and r-metHuSCF 20 microg/kg daily. Two PBPC collections were performed on consecutive days starting the day the WBC count was above 7.5 x 10(3)/microl. Collection was performed between days +9 and +12 and the median number of CD34(+) cells collected was 9.9 x 10(6)/kg (1.1-53.1) and 6.6 x 10(6)/kg (1.4-33.8) for the first and second apheresis, respectively. Despite being previously treated patients, the target CD34(+) cell dose required for SCT was obtained in all patients. SCT was associated with rapid neutrophil and platelet engraftment and a highly significant correlation was observed between the number of CD34(+) cells infused and engraftment. Treatment with SCF plus filgrastim was well tolerated, with mild to moderate local skin rash being the most frequently reported adverse event. In conclusion, addition of r-metHuSCF induces mobilization of a large number of CD34(+) cells which results in shortening of time to engraftment and hospitalization.

• Authors: F Prósper, C Solá, J Hornedo, C Arbona, P Menéndez, A Orfao, A Lluch, H Cortés-Funes, J J López, J García-Conde
• Journal: Leukemia (Leukemia) Vol. 17 Issue 2 Pg. 437-41 (Feb 2003) ISSN: 0887-6924 [Print] England
• PMID: 12592344 (Publication Type: Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Recombinant Proteins
• Stem Cell Factor
• Granulocyte Colony-Stimulating Factor
• Cyclophosphamide
• Filgrastim
• ancestim

Topics

• Adult
• Aged
• Breast Neoplasms (drug therapy, pathology, therapy)
• Cyclophosphamide (therapeutic use)
• Female
• Filgrastim
• Granulocyte Colony-Stimulating Factor (therapeutic use)
• Hematopoietic Stem Cell Mobilization (methods)
• Hematopoietic Stem Cells (drug effects, pathology)
• Humans
• Lymphatic Metastasis
• Middle Aged
• Neoplasm Metastasis
• Neoplasm Staging
• Recombinant Proteins (therapeutic use)
• Stem Cell Factor (analogs & derivatives, therapeutic use)
• Stem Cell Transplantation (adverse effects, methods)
• Transplantation, Autologous
• Treatment Outcome