Abstract | OBJECTIVES: METHODS: A total of 96 consecutive primary biliary cirrhosis patients and 283 pathologic controls were studied. To dissect the fine antinuclear antibodies specificities we used different techniques, such as indirect immunofluorescence on cryostat tissue sections and cell culture (HEp-2 cells), counterimmunoelectrophoresis with thymus and spleen extracts, ELISA assays with recombinant Sp100 and purified gp210 and Lamin B receptor, and immunoblot with several recombinant nuclear and cytoplasmic antigens. RESULTS:
Antinuclear antibodies were detected in 53% of patients, with the following hierarchy of specificities: 27% anti-Sp100, 16% "multiple nuclear dots," 16% anti-gp210, 16% anti-centromere, 7% XR1, 6% anti- lamin B receptor, 5% anti-SS-A/Ro, 5% anti- ribonucleoprotein, 4% XR2, 2% anti-SS-B/La, 2% perinuclear antineutrophil cytoplasmic antibodies, and 1% anti-double-stranded deoxyribonucleic acid. Several patients showed multiple specificities. The "multiple nuclear dots" pattern was detected more often in antimitochondrial antibodies negative patients. In particular, primary biliary cirrhosis specific antinuclear antibodies (anti-Sp100, anti-gp210, and anti- lamin B receptor) were detected in nine of 13 antimitochondrial negative primary biliary cirrhosis cases. Anti-gp210 was more frequent in patients with more pronounced cholestasis and more impaired liver function. CONCLUSIONS:
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Authors | Paolo Muratori, Luigi Muratori, Rodolfo Ferrari, Fabio Cassani, Giampaolo Bianchi, Marco Lenzi, Luis Rodrigo, Antonio Linares, Dolores Fuentes, Francesco B Bianchi |
Journal | The American journal of gastroenterology
(Am J Gastroenterol)
Vol. 98
Issue 2
Pg. 431-7
(Feb 2003)
ISSN: 0002-9270 [Print] United States |
PMID | 12591064
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Antibodies, Antinuclear
(analysis)
- Antibody Specificity
- Counterimmunoelectrophoresis
- Enzyme-Linked Immunosorbent Assay
- Female
- Fluorescent Antibody Technique, Indirect
- Humans
- Immunoblotting
- Liver Cirrhosis, Biliary
(immunology)
- Male
- Middle Aged
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