Identification of immunorelevant genes from greater wax moth (Galleria mellonella) by a subtractive hybridization approach.

Abstract

In this study we have analyzed bacterial lipopolysaccharide (LPS) induced genes in hemocytes of the Lepidopteran species Galleria mellonella using subtractive hybridization, followed by suppressive PCR. We have found genes that show homologies to molecules, such as gloverin, peptidoglycan recognition proteins and transferrin known to be involved in immunomodulation after bacterial infection in other species. In addition, a few molecules previously not described in the innate immune reactions were detected, such as a RNA binding molecule and tyrosine hydroxylase. Furthermore, the full-length cDNA of a LPS-induced molecule with six toxin-2-like domains is described to be a promising candidate to further elucidate the relationship between toxin- and defensin-like domains in arthropod host defense.

• Authors: V Seitz, A Clermont, M Wedde, M Hummel, A Vilcinskas, K Schlatterer, L Podsiadlowski
• Journal: Developmental and comparative immunology (Dev Comp Immunol) Vol. 27 Issue 3 Pg. 207-15 (Mar 2003) ISSN: 0145-305X [Print] United States
• PMID: 12590972 (Publication Type: Journal Article)

Chemical References

• Insect Proteins
• Lipopolysaccharides

Topics

• Amino Acid Sequence
• Animals
• Base Sequence
• Genes, Insect
• Immunity, Innate
• Insect Proteins (chemistry, genetics)
• Lipopolysaccharides (pharmacology)
• Molecular Sequence Data
• Moths (genetics, immunology)
• Nucleic Acid Hybridization
• Polymerase Chain Reaction