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[Mechanism of apoptosis induced by squamocin in leukemia cells].

AbstractAIM:
To investigate the mechanism of apoptosis of HL60 cells induced by the annonaceous acetogenin, squamocin.
METHODS:
Induction of apoptosis was determined through Hoechst33258 dye staining and DNA agarose gel electrophoresis. Expression of the proteins was detected using Western blot analysis. Caspase-3 activity was detected using caspase-3 kit.
RESULTS:
Treatment of HL-60 cells with squamocin resulted in extensive nuclear condensation, DNA fragmentation, cleavage of the death substrate poly(ADP-ribose) polymerase (PARP) and induction of caspase-3 activity. Pretreatment of HL-60 cells with caspase-3 specific inhibitor DEVD-CHO prevented squamocin-induced DNA fragmentation, PARP cleavage and cell death. Stress-activated protein kinase (SAPK/JNK) was activated after treatment with squamocin in HL-60 cells.
CONCLUSION:
These results suggest that apoptosis of HL-60 cells induced by squamocin require caspase-3 activation, and could be related to SAPK activation.
AuthorsX F Zhu, B F Xie, Z M Li, G K Feng, Y X Zeng, Z C Liu
JournalYao xue xue bao = Acta pharmaceutica Sinica (Yao Xue Xue Bao) Vol. 36 Issue 7 Pg. 498-501 (Jul 2001) ISSN: 0513-4870 [Print] China
PMID12585079 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antineoplastic Agents, Phytogenic
  • Drugs, Chinese Herbal
  • Furans
  • Lactones
  • squamocin
  • CASP3 protein, human
  • Caspase 3
  • Caspases
Topics
  • Annona (chemistry)
  • Antineoplastic Agents, Phytogenic (isolation & purification, pharmacology)
  • Apoptosis
  • Caspase 3
  • Caspases (metabolism)
  • Drugs, Chinese Herbal (isolation & purification, pharmacology)
  • Furans (isolation & purification, pharmacology)
  • HL-60 Cells
  • Humans
  • Lactones (isolation & purification, pharmacology)
  • Plants, Medicinal (chemistry)

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