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Bax, Bcl-2, fas and Fas-L antigen expression in human seminoma: correlation with the apoptotic index.

Abstract
Apoptosis plays a crucial role in the regulation of spermatogenesis in male germ cells and is, at least in part, modulated by Bcl-2, Bax, and the Fas pathway. Seminomas have a favourable outcome and respond to radio-/chemotherapy with an increased rate of apoptosis. The expression of Bax, Bcl-2, Fas and Fas-ligand (Fas-L) in human seminoma was evaluated and correlated with the apoptotic index. Twenty-nine classical seminomas were examined by immunohistochemistry and Western blotting using antibodies against Bax, Bcl-2, Fas and Fas-L. Apoptosis was detected by in-situ end-labeling of fragmented DNA and the apoptotic index (AI) was determined. Expression of Fas was found in 26 (89.7%) of Fas-L in 24 seminomas (82.2%); none of the tumours expressed Bcl-2. No correlation between the AI and Fas, Fas-L or Bcl-2 expression was found. Bax was demonstrated in 20/29 tumours (69%). Bax-positive tumours showed an increased AI of 4.75 +/- 2.38% in contrast to 2.60 +/- 1.23% of the Bax-negative tumours (P = 0.002). The number of Bax-positive tumour cells and apoptotic cells revealed a significant correlation using chi2-test (P = 0.04) and linear regression (r = 0.54, P = 0.001). Therefore, Bax seems to play a determinant role in the modulation of apoptosis in human seminoma that may be linked to a favourable outcome.
AuthorsRainer Grobholz, Hanswalter Zentgraf, Kai-Uwe Köhrmann, Uwe Bleyl
JournalAPMIS : acta pathologica, microbiologica, et immunologica Scandinavica (APMIS) Vol. 110 Issue 10 Pg. 724-32 (Oct 2002) ISSN: 0903-4641 [Print] Denmark
PMID12583439 (Publication Type: Journal Article)
Chemical References
  • BAX protein, human
  • FASLG protein, human
  • Fas Ligand Protein
  • Membrane Glycoproteins
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • bcl-2-Associated X Protein
  • fas Receptor
Topics
  • Adult
  • Apoptosis
  • Fas Ligand Protein
  • Humans
  • Immunohistochemistry
  • Male
  • Membrane Glycoproteins (metabolism)
  • Middle Aged
  • Proto-Oncogene Proteins (metabolism)
  • Proto-Oncogene Proteins c-bcl-2 (metabolism)
  • Seminoma (immunology, metabolism, pathology)
  • Testicular Neoplasms (immunology, metabolism, pathology)
  • bcl-2-Associated X Protein
  • fas Receptor (metabolism)

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