Endotoxin decreases serum IGFBP-3 and liver IGFBP-3 mRNA: comparison between Lewis and Wistar rats.

Abstract

We studied the IGFBP-3 response to endotoxin, in Wistar and Lewis rats. Compared to Wistar rats, Lewis rats have a reduced adrenal and IGF-I response to inflammatory stimuli. Rats received two injections of 1 mg/kg of lipopolysaccharide (LPS) and were killed 4 h after the second injection. LPS decreased serum concentrations of GH in Wistar (P<0.05), but not in Lewis rats. However, serum IGFBP-3 was decreased both in Wistar and in Lewis rats. Furthermore, LPS administration decreased IGFBP-3 gene expression in the liver in both rat strains (P<0.01). Lewis rats had lower serum IGFBP-3 than Wistar rats (P<0.01). This difference could be secondary to the increased IGFBP-3 proteolysis in serum observed in Lewis rats. These data indicate that acute inflammation inhibits serum concentrations of IGFBP-3 by decreasing its synthesis in the liver, rather than increasing its proteolysis. This effect seems to be GH and IGF-I independent.

• Authors: T Priego, I Ibáñez de Cáceres, A I Martín, M A Villanúa, A López-Calderón
• Journal: Molecular and cellular endocrinology (Mol Cell Endocrinol) Vol. 199 Issue 1-2 Pg. 23-8 (Jan 31 2003) ISSN: 0303-7207 [Print] Ireland
• PMID: 12581876 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Insulin-Like Growth Factor Binding Protein 3
• Lipopolysaccharides
• RNA, Messenger
• Endopeptidases

Topics

• Animals
• Endopeptidases (metabolism)
• Inflammation (blood)
• Insulin-Like Growth Factor Binding Protein 3 (biosynthesis, blood, drug effects)
• Lipopolysaccharides (pharmacology)
• Liver (metabolism)
• Male
• RNA, Messenger (biosynthesis, drug effects)
• Rats
• Rats, Inbred Lew (metabolism)
• Rats, Wistar (metabolism)