[Analysis of tannins in Fructus Chebulae and its confusion varieties by HPCE].

Abstract

AIM:

To analyze the hydrolyzable tannins-chebulinic acid (I) and chebulagic acid(II) in Fructus Chebulae and its confusion varieties by using high performance capillary electrophoresis (HPCE) method.

METHODS:

Using a capillary (375 microns OD x 50 microns ID; 81.5 cm x 61.5 cm) and a power supply set at 24 kV, with phosphate-borate buffer containing 20 mmol.L-1 Na2HPO4-60 mmol.L-1 boric acid and a UV detector at 280 nm, sample solution was loaded in decompression mode at the positive end of the capillary, the loading time was 5 s.

RESULTS:

The linear ranges of I and II were 0.0842-0.842 and 0.842 and 0.0940-0.940 mg.mL-1 respectively, the correlation coefficient were 0.9966 and 0.9957, the average recoveries were 95.6% (RSD = 4.0%, n = 5) and 95.0% (RSD = 4.4%, n = 5), the RSDs (n = 5) of measurement precision test were 2.2% and 1.7%, the RSDs (n = 6) of reproduction test were 5.4% and 4.0% respectively. The contents of I and II were obviously interrelated with the variety and characteristics of Fructus Chebulae, the contents of I and II in the confusion varieties of Fructus Chebulae were very low.

CONCLUSION:

It is suitable to use I and II as the criterion in quality evaluation of Fructus Chebulae, and the HPCE method is effective for quality evaluation of the crude Fructus Chebulae.

• Authors: G Ding, Y R Lu, C R Ji, Y Z Liu
• Journal: Yao xue xue bao = Acta pharmaceutica Sinica (Yao Xue Xue Bao) Vol. 36 Issue 4 Pg. 292-5 (Apr 2001) ISSN: 0513-4870 [Print] China
• PMID: 12580059 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Benzopyrans
• Glucosides
• Hydrolyzable Tannins
• Tannins
• chebulinic acid
• chebulagic acid

Topics

• Benzopyrans (analysis)
• Drug Contamination
• Electrophoresis, Capillary
• Fruit (chemistry)
• Glucosides (analysis)
• Hydrolyzable Tannins
• Plants, Medicinal (chemistry, classification)
• Quality Control
• Tannins (analysis)
• Terminalia (chemistry, classification)