[Effects of praeruptorin C on cell hypertrophy, intracellular [Ca2+]i, nitric oxide and signal transduction in isolated hypertrophied rat smooth muscle cells induced by angiotensin II].

Abstract

AIM:

To investigate the effects of praeruptorin C (Pra-C) on smooth muscle cell (SMC) hypertrophy, intracellular calcium ([Ca2+]i), nitric oxide (NO) content and influence on cellular signal transduction in isolated cultured rat smooth muscle cell (SMC).

METHODS:

Hypertrophied smooth muscle cells (HSMCs) were induced by angiotensin II (Ang II), cell area was measured under inverted microscope. Nitric oxide (NO) concentration was measured using Griess method. [Ca2+]i was measured using Fura-2/AM. The responses to [Ca2+]i elevation stimulated by KCl (60 mmol.L-1 or norepinephrine (10 mumol.L-1) were observed by incubation with phorbol 12-myristate 13-acetate (PMA), staurosporine (ST), the agonist and inhibitor of protein kinase C (PKC), and pertussis toxin (PTX), the sensitive toxin of Gi.

RESULTS:

The cell area of SMCs were decreased by 39.01% (P < 0.001) and NO content of SMCs were significantly increased in Pra-C + Ang II group. In presence of 60 mmol.L-1 KCl or 10 mumol.L-1 NE, [Ca2+]i of SMCs in Pra-C + Ang II group was significantly decreased than that of Ang II group (P < 0.001) and closed to the normal group. Incubation of SMCs with PMA, ST and PTX, [Ca2+]i of SMCs in Ang II group was increased by PMA and decreased by ST and PTX, but that of Pra-C + Ang II group was similar to the normal group.

CONCLUSION:

These findings suggest that Pra-C can reduce vascular hypertrophy in isolated rat HSMCs, and this is associated with improvement of SMCs [Ca2+]i level, NO content and cellular signal transdution of PKC and Gi.

• Authors: Man-ren Rao, Wan-bin Liu, Xiao-wen Zhang
• Journal: Yao xue xue bao = Acta pharmaceutica Sinica (Yao Xue Xue Bao) Vol. 37 Issue 1 Pg. 5-9 (Jan 2002) ISSN: 0513-4870 [Print] China
• PMID: 12579890 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Calcium Channel Blockers
• Coumarins
• Angiotensin II
• Nitric Oxide
• praeruptorin C
• Calcium

Topics

• Angiotensin II
• Animals
• Aorta (pathology)
• Calcium (metabolism)
• Calcium Channel Blockers (pharmacology)
• Cells, Cultured
• Coumarins (pharmacology)
• Female
• Hypertrophy (chemically induced, pathology)
• Male
• Muscle, Smooth, Vascular (drug effects)
• Myocytes, Smooth Muscle (drug effects, pathology)
• Nitric Oxide (metabolism)
• Rats
• Rats, Sprague-Dawley
• Signal Transduction (drug effects)