Encephalitogenic activity of truncated myelin oligodendrocyte glycoprotein (MOG) peptides and their recognition by CD8+ MOG-specific T cells on oligomeric MHC class I molecules.

Abstract

We have previously demonstrated that the 21-residue peptide pMOG(35-55) from myelin oligodendrocyte glycoprotein (MOG) contains an antigenic epitope that activates CD8(+) encephalitogenic T cells in C57BL/6 (B6) mice. To identify the core encephalitogenic epitope of CD8(+) MOG-specific T cells, we have prepared a panel of highly purified peptides of varying lengths, which span the entire length of pMOG(35-55), and tested their binding to recombinant H-2D(b) dimers and their ability to induce EAE. Two of the truncated peptides, pMOG(40-54) and pMOG(44-54), strongly bound recombinant H-2D(b) protein and this complex bound MOG-specific CD8(+) T cells. Interestingly, pMOG(40-54) retained the full capability of inducing paralytic disease, whereas only a part of the B6 mice immunized with pMOG(44-54) developed clinical paralysis and central nervous system (CNS) inflammation. Further deletion of 1 amino acid from either the N- or C-terminus of the peptide pMOG(44-54) dramatically reduced binding to recombinant H-2D(b), and abolished the induction of paralysis and CNS inflammation. Our results demonstrate that the ability of truncated pMOG(35-55) peptides to bind recombinant H-2D(b) protein does not always correlate with their ability of inducing encephalomyelitis. This approach enables the further identification of the core pathogenic epitope within the pMOG(35-55) that activates MOG-specific encephalitogenic CD8(+) T cells.

• Authors: Deming Sun, Yiping Zhang, Bingyuan Wei, Stephen C Peiper, Hui Shao, Henry J Kaplan
• Journal: International immunology (Int Immunol) Vol. 15 Issue 2 Pg. 261-8 (Feb 2003) ISSN: 0953-8178 [Print] England
• PMID: 12578856 (Publication Type: Journal Article)

Chemical References

• Histocompatibility Antigens Class I
• Mog protein, mouse
• Myelin Proteins
• Myelin-Associated Glycoprotein
• Myelin-Oligodendrocyte Glycoprotein
• Peptides

Topics

• Animals
• CD8-Positive T-Lymphocytes (metabolism)
• Encephalitis (metabolism)
• Histocompatibility Antigens Class I (metabolism)
• Hybridomas (metabolism)
• Mice
• Myelin Proteins
• Myelin-Associated Glycoprotein (metabolism)
• Myelin-Oligodendrocyte Glycoprotein
• Peptides (metabolism)