[Application of dot immunogold filtration assay for detecting serum antibodies in clonorchiasis patients].

Abstract

OBJECTIVE:

To establish a new, fast, sensitive and specific assay for detecting antibodies in clonorchiasis.

METHODS:

Using Clonorchis sinensis adult worm antigen and SPA labelled with colloidal gold as color developing agent, a dot immunogold filtration assay (DIGFA) for detecting clonorchiasis antibodies was tested. Dot-ELISA was used as the parallel control.

RESULTS:

The positive rate of DIGFA and dot-ELISA in testing the sera from 119 proved cases were 96.4% (115/119) and 92.4% (110/119), respectively, the difference between the two assays being in significant (P > 0.05). The negative rate of DIGFA in healthy people was 100% (40/40). The cross reaction rate in 20 cysticercosis cases and 25 schistosomiasis cases were 5% (1/20) and 4% (1/25), respectively. Both coincidence rates comparing DIGFA with dot-ELISA were 90.9% (50/55).

CONCLUSION:

DIGFA is as sensitive and specific as the dot-ELISA, and has the advantages of simplicity and without specific equipment.

• Authors: D Y Liu, W Q Hu, H M Zhang
• Journal: Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi) Vol. 19 Issue 2 Pg. 97-9 ( 2001) ISSN: 1000-7423 [Print] China
• PMID: 12571996 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Helminth

Topics

• Animals
• Antibodies, Helminth (blood)
• Clonorchiasis (diagnosis, immunology)
• Clonorchis sinensis (immunology)
• Enzyme-Linked Immunosorbent Assay
• Humans
• Immunoblotting (methods)
• Sensitivity and Specificity