Determination of Tripamide in human urine by high-performance liquid chromatography and high-performance liquid chromatography/electrospray ionization tandem mass spectrometry.

Abstract

Tripamide is a drug widely used in clinical practice for the treatment of hypertension and edema. This work evaluated a screening method for Tripamide and its urinary metabolites in human urine, using high-performance liquid chromatography diode-array detection (HPLC/DAD). Identification of these metabolites was investigated by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC/ESI-MS/MS) after dosing with 15 mg Tripamide. Acid hydrolysis showed that Tripamide is conjugated in the body. Two suspected metabolites were detected by HPLC/DAD. HPLC/ESI-MS/MS analysis suggested that these metabolites were probably hydroxylated together with loss of the -NH(2) group and dehydrogenation. These results will be useful in confirmation methods for Tripamide in doping control.

• Authors: Yunje Kim, Joungboon Hwang, Myungsoo Kim, Won Lee
• Journal: Rapid communications in mass spectrometry : RCM (Rapid Commun Mass Spectrom) Vol. 17 Issue 4 Pg. 301-6 ( 2003) ISSN: 0951-4198 [Print] England
• PMID: 12569439 (Publication Type: Journal Article)
• Copyright: Copyright 2003 John Wiley & Sons, Ltd.

Chemical References

• Diuretics
• Indoles
• tripamide

Topics

• Adult
• Chromatography, High Pressure Liquid (methods)
• Diuretics (chemistry, metabolism, urine)
• Humans
• Indoles (chemistry, metabolism, urine)
• Male
• Molecular Structure
• Spectrometry, Mass, Electrospray Ionization (methods)
• Urinalysis (methods)