[Evaluation of Schistosoma japonicum tegumental membrane antigen (SjMAg) in immunodiagnosis and efficacy assessment].

Abstract

OBJECTIVE:

To evaluate the specificity and sensitivity of SjMAg in detecting specific antibodies in sera of patients with schistosomiasis japonica and in assessing therapeutic efficacy.

METHODS:

SjMAg-ELISA was used to determine the specific IgG and IgG4 in sera of patients with schistosomiasis japonica at different time points after chemotherapy.

RESULTS:

SjMAg-ELISA was as sensitive and specific as SEA-ELISA. The negative conversion rate of IgG detected by SjMAg-ELISA 12 months after treatment was 80.0%, being significantly higher than 43.3% by SEA-ELISA. The negative conversion rate of IgG4 detected by SjMAg-ELISA 12 months after treatment was 93.3%, being significantly higher than 60.0% by SEA-ELISA. The negative conversion rates of IgG and IgG4 2 yr after treatment were 92.9% and 97.6% respectively.

CONCLUSION:

SjMAg-ELISA is comparable to SEA-ELISA in diagnosing schistosomiasis japonica and is more effective in assessing therapeutic efficacy.

• Authors: S K Zhang, X Y Yi, X H Shu, Z J Li, Q R Zeng, J C Zhou, X F Zeng
• Journal: Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi) Vol. 18 Issue 2 Pg. 69-72 ( 2000) ISSN: 1000-7423 [Print] China
• PMID: 12567716 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Helminth
• Antigens, Helminth
• Immunoglobulin G

Topics

• Animals
• Antibodies, Helminth (blood)
• Antigens, Helminth (immunology)
• Enzyme-Linked Immunosorbent Assay (methods)
• Humans
• Immunoglobulin G (blood)
• Schistosoma japonicum (immunology)
• Schistosomiasis japonica (diagnosis)
• Sensitivity and Specificity