Dose-dependent protective effect of selenium in rat model of Parkinson's disease: neurobehavioral and neurochemical evidences.

Abstract

Normal cellular metabolism produces oxidants that are neutralized within cells by antioxidant enzymes and other antioxidants. An imbalance between oxidant and antioxidant has been postulated to lead the degeneration of dopaminergic neurons in Parkinson's disease. In this study, we examined whether selenium, an antioxidant, can prevent or slowdown neuronal injury in a 6-hydroxydopamine (6-OHDA) model of Parkinsonism. Rats were pre-treated with sodium selenite (0.1, 0.2 and 0.3 mg/kg body weight) for 7 days. On day 8, 2 micro L 6-OHDA (12.5 micro g in 0.2% ascorbic acid in normal saline) was infused in the right striatum. Two weeks after 6-OHDA infusion, rats were tested for neurobehavioral activity, and were killed after 3 weeks of 6-OHDA infusion for the estimation of glutathione peroxidase, glutathione-S-transferase, glutathione reductase, glutathione content, lipid peroxidation, and dopamine and its metabolites. Selenium was found to be successful in upregulating the antioxidant status and lowering the dopamine loss, and functional recovery returned close to the baseline dose-dependently. This study revealed that selenium, which is an essential part of our diet, may be helpful in slowing down the progression of neurodegeneration in parkinsonism.

• Authors: Khan Shoeb Zafar, Almas Siddiqui, Iqbal Sayeed, Muzamil Ahmad, Sofian Salim, Fakhrul Islam
• Journal: Journal of neurochemistry (J Neurochem) Vol. 84 Issue 3 Pg. 438-46 (Feb 2003) ISSN: 0022-3042 [Print] England
• PMID: 12558963 (Publication Type: Journal Article)

Chemical References

• Antioxidants
• Neuroprotective Agents
• 3,4-Dihydroxyphenylacetic Acid
• Glutathione
• Sodium Selenite
• Ascorbic Acid
• Dopamine
• Homovanillic Acid

Topics

• 3,4-Dihydroxyphenylacetic Acid (metabolism)
• Animals
• Antioxidants (metabolism)
• Ascorbic Acid (administration & dosage, metabolism)
• Behavior, Animal (drug effects)
• Brain (drug effects, metabolism, physiopathology)
• Disease Models, Animal
• Dopamine (metabolism)
• Dose-Response Relationship, Drug
• Drug Administration Routes
• Drug Administration Schedule
• Glutathione (metabolism)
• Homovanillic Acid (metabolism)
• Lipid Peroxidation (drug effects)
• Male
• Motor Activity (drug effects)
• Neuroprotective Agents (administration & dosage, therapeutic use)
• Parkinsonian Disorders (chemically induced, physiopathology, prevention & control)
• Rats
• Rats, Wistar
• Recovery of Function
• Sodium Selenite (administration & dosage, therapeutic use)
• Treatment Outcome