Abstract |
To understand further the molecular basis of rickettsial host cell invasion, Rickettsia prowazekii invasion gene homolog (invA) has been characterized. Our previous experiments have shown that InvA is an Ap5A pyrophosphatase, a member of the Nudix hydrolase family, which is up-regulated during the internalization, early growth phase, and exit steps during rickettsial mammalian cell infection. In addition to the molecular characterization, subcellular localization of InvA was investigated. InvA-specific antibodies were raised in mice and used for immunoelectron microscopy. The generated antibodies were shown to recognize InvA and by immunogold labeling showed InvA in the cytoplasm of rickettsiae. A cytoplasmic location for InvA would allow for a rapid response to any internal substance and efficient functioning in hydrolysis of toxic metabolic by-products that are accumulated in the rickettsial cytoplasm during host cell invasion. Protecting bacteria from a hazardous environment could enhance their viability and allow them to remain metabolically active, which is a necessary step for the rickettsial obligate intracellular lifestyle.
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Authors | Jariyanart Gaywee, John B Sacci Jr, Suzana Radulovic, Magda S Beier, Abdu F Azad |
Journal | The American journal of tropical medicine and hygiene
(Am J Trop Med Hyg)
Vol. 68
Issue 1
Pg. 92-6
(Jan 2003)
ISSN: 0002-9637 [Print] United States |
PMID | 12556155
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Bacterial Proteins
- invA protein, Bacteria
- Pyrophosphatases
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Topics |
- Animals
- Bacterial Proteins
(analysis)
- Blotting, Western
- Chlorocebus aethiops
- Electrophoresis, Polyacrylamide Gel
- Enzyme-Linked Immunosorbent Assay
- Female
- Fluorescent Antibody Technique, Indirect
- Mice
- Mice, Inbred BALB C
- Microscopy, Immunoelectron
- Pyrophosphatases
(analysis)
- Rickettsia prowazekii
(chemistry, enzymology, ultrastructure)
- Rickettsia typhi
(chemistry, enzymology, ultrastructure)
- Vero Cells
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