Identification of a homologue of a house dust mite allergen in a cDNA library from Sarcoptes scabiei var hominis and evaluation of its vaccine potential in a rabbit/S. scabiei var. canis model.

Abstract	Sarcoptes scabiei ("itch mite") causes scabies, a disease of considerable human and veterinary significance. Little work has been done at the molecular level because of the difficulty of obtaining mites. We have used mites in skin from the bedding of crusted scabies patients for the construction of a library of 10(5) cDNAs from S. scabiei var. hominis cloned in the vector pGEX4T-2. We describe the isolation by immunoscreening of 2 clones, one of which (Ssagl) is homologous to and cross-reactive with the house dust mite Euroglyphus maynei allergen M-177, an apolipoprotein from hemolymph. Immunohistochemistry revealed that it is located around the internal organs and cuticle of the mite and in eggs. Although it was not found to be protective in a challenge trial, the rabbits did not exhibit typical crust characteristics. This work shows that it is now possible to conduct such challenge trials with cloned scabies antigens.
Authors	Pearly Harumal, Marjorie Morgan, Shelley F Walton, Deborah C Holt, Jurgen Rode, Larry G Arlian, Bart J Currie, David J Kemp
Journal	The American journal of tropical medicine and hygiene (Am J Trop Med Hyg) Vol. 68 Issue 1 Pg. 54-60 (Jan 2003) ISSN: 0002-9637 [Print] United States
PMID	12556149 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References	Allergens Antigens DNA, Complementary Immune Sera RNA, Messenger Vaccines
Topics	Allergens (chemistry, genetics, immunology) Amino Acid Sequence Animals Antigens (chemistry, genetics, immunology) Base Sequence Cloning, Molecular DNA, Complementary (chemistry, genetics) Disease Models, Animal Gene Library Humans Immune Sera (immunology) Immunohistochemistry Molecular Sequence Data Polymerase Chain Reaction Pyroglyphidae (genetics, immunology) RNA, Messenger (genetics) Rabbits Sarcoptes scabiei (genetics, immunology) Scabies (immunology, parasitology, prevention & control) Sequence Alignment Sequence Homology, Amino Acid Vaccines (genetics, immunology)

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