Abstract | BACKGROUND: METHODS: Sputum induction was performed in 30 patients with COPD and 15 normal control subjects. Levels of nitrogen oxides, percentage of neutrophils, and interleukin 8 (IL-8) levels were measured in sputum samples, and peroxynitrite inhibitory activity was assayed by monitoring rhodamine formation. RESULTS:
Nitrite and nitrate levels in induced sputum were significantly higher in patients with COPD than in normal controls (949 (133) microM v 621 (89) microM, p<0.001). In contrast, peroxynitrite inhibitory activity in induced sputum was significantly lower in patients with COPD than in normal controls (47.4 (12.7)% v 92.9 (3.9)%, p<0.001). There was a negative correlation between nitrite and nitrate levels and peroxynitrite inhibitory activity in induced sputum (r=-0.775, p<0.001). Peroxynitrite inhibitory activity was also significantly correlated with forced expiratory volume in 1 second (FEV(1)) % predicted (r=0.539, p=0.004), FEV(1)/FVC (r=0.512, p=0.006), and carbon monoxide transfer factor (TLCO) (r=0.486, p=0.009). Moreover, there was a significant negative correlation between peroxynitrite inhibitory activity and the degree of neutrophilic inflammation (percentage of neutrophils: r=-0.754, p<0.001; IL-8 levels: r=-0.497, p=0.007). CONCLUSIONS:
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Authors | H Kanazawa, S Shiraishi, K Hirata, J Yoshikawa |
Journal | Thorax
(Thorax)
Vol. 58
Issue 2
Pg. 106-9
(Feb 2003)
ISSN: 0040-6376 [Print] England |
PMID | 12554889
(Publication Type: Clinical Trial, Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)
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Chemical References |
- Interleukin-8
- Nitrogen Oxides
- Peroxynitrous Acid
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Topics |
- Female
- Forced Expiratory Volume
(physiology)
- Humans
- Interleukin-8
(metabolism)
- Male
- Middle Aged
- Neutrophils
(metabolism)
- Nitrogen Oxides
(metabolism)
- Peroxynitrous Acid
(metabolism)
- Pulmonary Disease, Chronic Obstructive
(metabolism)
- Sputum
(metabolism)
- Vital Capacity
(physiology)
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