Abstract | BACKGROUND: METHODS:
Sodium channel function was assayed using patch-clamp recordings, both in heterologous expression systems and in intact neurons. RESULTS: QYNAD synthesized in 10 different batches by four different facilities failed to block sodium currents, even at concentrations as high as 500 micro M (50-fold higher than the blocking concentration originally reported). QYNAD had no effect on the currents produced by recombinant Na(v)1.2, Na(v)1.4, Na(v)1.6, and Na(v)1.7 sodium channels or on the sodium currents that are produced by native channels in adult hippocampal or dorsal root ganglion neurons. QYNAD did not interfere with conduction in the optic nerve, a myelinated fiber tract that is often affected in MS. CONCLUSIONS:
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Authors | T R Cummins, M Renganathan, P K Stys, R I Herzog, K Scarfo, R Horn, S D Dib-Hajj, S G Waxman |
Journal | Neurology
(Neurology)
Vol. 60
Issue 2
Pg. 224-9
(Jan 28 2003)
ISSN: 1526-632X [Electronic] United States |
PMID | 12552035
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Oligopeptides
- Recombinant Proteins
- Sodium Channels
- pentapeptide QYNAD
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Topics |
- Animals
- CHO Cells
- Cells, Cultured
- Cricetinae
- Dose-Response Relationship, Drug
- Ganglia, Spinal
(cytology)
- Humans
- In Vitro Techniques
- Male
- Mice
- Neurons
(cytology, drug effects, metabolism)
- Oligopeptides
(pharmacology)
- Oocytes
(cytology, drug effects, metabolism)
- Optic Nerve
(drug effects, physiology)
- Patch-Clamp Techniques
- Rats
- Rats, Long-Evans
- Rats, Sprague-Dawley
- Recombinant Proteins
(drug effects, genetics, metabolism)
- Sodium Channels
(drug effects, genetics, metabolism)
- Transfection
- Xenopus
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