The replication of rodent parvovirus X14.

Abstract

The replication of rodent parvovirus X14 DNA has been studied in rat embryo tissue culture cells. Virus DNA was isolated from I M-NaCl-SDS-pronase supernatant fluids from 24 h after infection. The majority of this DNA was 1-7 mum in length and double-stranded, indicating that it was an intermediate in the replication cycle of this single-stranded DNA virus. Single-stranded DNA of equivalent length was isolated directly from X14 virions. The buoyant density of this DNA was 1-728 g/ml whereas the double-stranded form banded at 1-714 g/ml in caesium chloride gradients. Difficulties in detecting significant amounts of single-stranded viral DNA directly from infected cells would appear to indicate that progeny single-stranded DNA is rapidly encapsidated after synthesis.

• Authors: H D Mayor, L E Jordan
• Journal: The Journal of general virology (J Gen Virol) Vol. 30 Issue 3 Pg. 337-46 (Mar 1976) ISSN: 0022-1317 [Print] England
• PMID: 1255136 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• DNA, Single-Stranded
• DNA, Viral

Topics

• Culture Techniques
• DNA Replication
• DNA, Single-Stranded (analysis, biosynthesis)
• DNA, Viral (analysis, biosynthesis)
• Parvoviridae (analysis, growth & development, metabolism)
• Virus Replication