Tumor-associated blocking factors: isolation from sera of tumor-bearing mice.

Abstract

An isolation and partial purifications of tumor-associated blocking factors from the sera of tumor-bearing mice is described. Columns for affinity chromatography were prepared by coupling syngeneic tumor-immune antibodies to Sepharose 4B. Passage of serum through such immunoadsorbent columns removed all blocking activity from tumor-bearers' sera; subsequent elution of the absorbent with 3 M NaSCN recovered the activity. The blocking material was further purified on Sephadex G-200. The data provide evidence for the presence of antigen in tumor-associated blocking factors and are compatible with the hypothesis that blocking factors often consist of antigen and antibodies in the form of immune complexes.

• Authors: J Tamerius, J Nepom, I Hellström, K E Hellström
• Journal: Journal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 116 Issue 3 Pg. 724-30 (Mar 1976) ISSN: 0022-1767 [Print] United States
• PMID: 1254951 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antigens, Neoplasm
• Immune Sera

Topics

• Adsorption
• Animals
• Antigens, Neoplasm
• Chromatography, Gel
• Immune Sera (pharmacology)
• Male
• Mice
• Mice, Inbred BALB C
• Sarcoma, Experimental (immunology)
• Time Factors