Nitric oxide and endothelium-derived hyperpolarizing factor in human arteries and veins.

Abstract

BACKGROUND:

We have investigated and compared nitric oxide (NO) release and endothelium-derived hyperpolarizing factor (EDHF)-mediated hyperpolarization in the human internal mammary artery (IMA), radial artery (RA), saphenous vein (SV), and coronary artery.

MATERIALS AND METHODS:

Vessel segments taken from coronary artery bypass grafting or heart transplantation patients were placed in an organ chamber. NO-sensitive electrode or intracellular glass microelectrode was used to study NO or EDHF in response to acetylcholine (ACh) and bradykinin (BK).

RESULTS:

The resting membrane potential of the smooth muscle cells of IMA, RA, and SV was -58 +/- 0.84 (n = 61), -61 +/- 1.3 mV (n = 46, p = 0.03), and -62 +/- 0.9 mV (n = 23, p = 0.0001) respectively. BK- (10(-7) M) induced EDHF-mediated hyperpolarization (-10.9 +/- 1.5 mV, n = 7) in the IMA was significantly greater than that in RA (-5.8 +/- 0.9 mV, n = 6, p = 0.04) and SV (-5.1 +/- 0.5 mV, n = 8, p < 0.01). The basal release of NO in IMA (16.8 +/- 1.9 nM) was significantly higher than that in RA (11.1 +/- 1.0 nM, n = 12, p = 0.02) and in SV (9.9 +/- 2.8 nM, n = 13, p < 0.001). The stimulated release of NO to BK in IMA was significantly greater than that in RA (44.3 +/- 4.0 vs 25.8 +/- 3.6 nM, n = 8, p = 0.004). The duration of NO release was longer in IMA than in RA or in SV.

CONCLUSIONS:

The basal and stimulated release of NO and EDHF-mediated hyperpolarization in the IMA are significantly greater than that in the RA and SV. EDHF exists in all these human vessels. This study reveals the differences among human vessels regarding the endothelial function that have implications in vasospasm, coronary protection, or long-term graft patency.

• Authors: Guo-Wei He
• Journal: Journal of cardiac surgery (J Card Surg) Vol. 17 Issue 4 Pg. 317-23 ( 2002) ISSN: 0886-0440 [Print] United States
• PMID: 12546079 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biological Factors
• Biomarkers
• Cardiovascular Agents
• endothelium-dependent hyperpolarization factor
• Nitric Oxide
• Indomethacin

Topics

• Biological Factors (metabolism)
• Biomarkers (blood)
• Cardiovascular Agents (pharmacology)
• Coronary Vessels (drug effects, metabolism)
• Electrophysiologic Techniques, Cardiac
• Endothelium, Vascular (chemistry)
• Humans
• Indomethacin (pharmacology)
• Mammary Arteries (drug effects, metabolism)
• Muscle, Smooth, Vascular (blood supply, metabolism)
• Nitric Oxide (metabolism)
• Radial Artery (drug effects, metabolism)
• Saphenous Vein (drug effects, metabolism)
• Vasodilation (drug effects, physiology)