Neuroprotective effects of R(-)-1-(benzo[b]thiophen-5-yl)-2- [2-(N,N-diethylamino)ethoxy]ethanol hydrochloride (T-588) against retinal ganglion cell death induced by elevated intraocular pressure in rat.

Abstract

PURPOSE:

We investigated whether T-588 can attenuate retinal ganglion cell (RGC) death induced by elevated intraocular pressure (IOP).

METHODS:

IOP elevation was induced unilaterally by argon laser irradiation of the rat trabecular meshwork 4 days after an intracameral injection of India ink. We orally administered either the vehicle, or 10, 30, or 100 mg/kg body weight (BW) of T-588 24 hours before the laser application. Five days after the laser application, 1.5 microL of 3% Fast Blue was injected into the superior colliculi bilaterally. Three days after the Fast Blue injection, the eye was enucleated and the retinal whole flatmount was prepared. Labeled ganglion cells were counted by fluorescence microscope with an ultraviolet filter.

RESULTS:

Laser treatment significantly increased the IOP. The percentages of labeled RGCs in the lasered eyes as compared with the nonlasered contralateral eyes were 78.0 +/- 11.6% in the control group, 78.7 +/- 12.9% in the 10 mg/kg BW group, 79.1 +/- 13.0% in the 30 mg/kg BW group, and 91.0 +/- 9.0% in the 100 mg/kg BW T-588-treated group. The survival rate of RGCs was significantly higher in the 100 mg/kg BW T-588-treated group than in the control group.

CONCLUSION:

T-588 appears to have a neuroprotective effect on retinal ganglion cells in this ocular hypertensive model.

• Authors: Md Zahidul Karim, Zhaobin Gu, Akira Sawada, Toru Taniguchi, Kazuhide Kawase, Tetsuya Yamamoto, Yoshiaki Kitazawa
• Journal: Japanese journal of ophthalmology (Jpn J Ophthalmol) 2002 Nov-Dec Vol. 46 Issue 6 Pg. 621-6 ISSN: 0021-5155 [Print] Japan
• PMID: 12543187 (Publication Type: Journal Article)

Chemical References

• Diethylamines
• Neuroprotective Agents
• Thiophenes
• T 588

Topics

• Administration, Oral
• Animals
• Cell Count
• Cell Death (drug effects)
• Diethylamines (pharmacology)
• Intraocular Pressure
• Laser Therapy
• Male
• Microscopy, Fluorescence
• Neuroprotective Agents (pharmacology)
• Ocular Hypertension (complications)
• Optic Nerve Diseases (etiology, pathology, prevention & control)
• Rats
• Rats, Wistar
• Retinal Ganglion Cells (drug effects, pathology)
• Thiophenes (pharmacology)
• Trabecular Meshwork (surgery)