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Cloning and expression analysis of rainbow trout Oncorhynchus mykiss interferon regulatory factor 1 and 2 (IRF-1 and IRF-2).

Abstract
Two rainbow trout (Oncorhynchus mykiss) genes for interferon regulatory factors (IRF) 1 and 2 have been cloned and sequenced. The IRF-1 cDNA contains an open reading frame (ORF) of 996 nucleotides that translates into a 331 amino-acid putative peptide, with a 5' untranslated region (UTR) of 145bp and a 3' UTR of 481bp. The IRF-2 cDNA contains a 1035bp ORF that translates into a 344 amino-acid putative peptide, with a 5' UTR of 146bp and a 3' UTR of 925bp. In vivo, IRF-1 and IRF-2 are constitutively expressed in head kidney, gill and spleen but not liver. Both genes were induced in all the tissues examined. IRF-1 but not IRF-2 expression was significantly increased at the site of injection 1 week after DNA vaccination against viral haemorrhagic septicaemia virus. In vitro, IRF-1 and IRF-2 transcripts are present in unstimulated rainbow trout gonad cells and are up-regulated by poly I/C.
AuthorsBertrand Collet, Guido C J Hovens, David Mazzoni, Ikuo Hirono, Takashi Aoki, Christopher J Secombes
JournalDevelopmental and comparative immunology (Dev Comp Immunol) Vol. 27 Issue 2 Pg. 111-26 (Feb 2003) ISSN: 0145-305X [Print] United States
PMID12543125 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • DNA-Binding Proteins
  • Interferon Regulatory Factor-1
  • Interferon Regulatory Factor-2
  • Phosphoproteins
  • Repressor Proteins
  • Transcription Factors
  • Vaccines, DNA
  • Viral Vaccines
Topics
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • DNA-Binding Proteins (chemistry, genetics)
  • Gene Expression
  • Interferon Regulatory Factor-1
  • Interferon Regulatory Factor-2
  • Molecular Sequence Data
  • Novirhabdovirus (immunology)
  • Oncorhynchus mykiss (immunology)
  • Phosphoproteins (chemistry, genetics)
  • Repressor Proteins
  • Transcription Factors
  • Vaccines, DNA (immunology)
  • Viral Vaccines (immunology)

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