Abstract |
Two rainbow trout (Oncorhynchus mykiss) genes for interferon regulatory factors (IRF) 1 and 2 have been cloned and sequenced. The IRF-1 cDNA contains an open reading frame (ORF) of 996 nucleotides that translates into a 331 amino-acid putative peptide, with a 5' untranslated region (UTR) of 145bp and a 3' UTR of 481bp. The IRF-2 cDNA contains a 1035bp ORF that translates into a 344 amino-acid putative peptide, with a 5' UTR of 146bp and a 3' UTR of 925bp. In vivo, IRF-1 and IRF-2 are constitutively expressed in head kidney, gill and spleen but not liver. Both genes were induced in all the tissues examined. IRF-1 but not IRF-2 expression was significantly increased at the site of injection 1 week after DNA vaccination against viral haemorrhagic septicaemia virus. In vitro, IRF-1 and IRF-2 transcripts are present in unstimulated rainbow trout gonad cells and are up-regulated by poly I/C.
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Authors | Bertrand Collet, Guido C J Hovens, David Mazzoni, Ikuo Hirono, Takashi Aoki, Christopher J Secombes |
Journal | Developmental and comparative immunology
(Dev Comp Immunol)
Vol. 27
Issue 2
Pg. 111-26
(Feb 2003)
ISSN: 0145-305X [Print] United States |
PMID | 12543125
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA-Binding Proteins
- Interferon Regulatory Factor-1
- Interferon Regulatory Factor-2
- Phosphoproteins
- Repressor Proteins
- Transcription Factors
- Vaccines, DNA
- Viral Vaccines
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Topics |
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Line
- Cloning, Molecular
- DNA-Binding Proteins
(chemistry, genetics)
- Gene Expression
- Interferon Regulatory Factor-1
- Interferon Regulatory Factor-2
- Molecular Sequence Data
- Novirhabdovirus
(immunology)
- Oncorhynchus mykiss
(immunology)
- Phosphoproteins
(chemistry, genetics)
- Repressor Proteins
- Transcription Factors
- Vaccines, DNA
(immunology)
- Viral Vaccines
(immunology)
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