The canine mast cell activation via CRP.

Abstract

We report here canine mastocytoma-derived cell (CMMC) activation via two pentraxin, limulus- and human-CRP. Mast cell chemotaxis was measured by Boyden's blindwell chamber. To confirm that the cell migration was chemotactic, "checkerboard" analysis was performed. We used Fura-2 to investigate CRP-mediated cytosolic calcium elevation. To examine whether CRP-induced stimulation is mediated through G-proteins, CMMC were incubated with pertussis toxin (PTx) before use in chemotaxis assay and Ca(2+) mobilization. CMMC migration in response to CRP was both chemokinetic and chemotactic. Limulus-CRP induced a transient Ca(2+)-mobilization dose-dependently. Preincubation of the cells with PTx inhibited CRP chemotaxis and Ca(2+)-mobilization, suggesting that G-proteins of the Gi-class are involved in the chemotaxis. We suggest that CRP may participate in the migration of mast cells to inflamed tissues during an acute-phase response. CRP-mediated recruitment of mast cells might play an important role in hypersensitivity and inflammatory processes.

• Authors: Takahiro Fujimoto, Yoshitaka Sato, Nobuo Sasaki, Reiko Teshima, Kazuo Hanaoka, Seiichi Kitani
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 301 Issue 1 Pg. 212-7 (Jan 31 2003) ISSN: 0006-291X [Print] United States
• PMID: 12535664 (Publication Type: Journal Article)

Chemical References

• Fluorescent Dyes
• Histamine
• C-Reactive Protein
• Pertussis Toxin
• Calcium
• Fura-2

Topics

• Animals
• C-Reactive Protein (pharmacology)
• Calcium (metabolism)
• Chemotaxis (physiology)
• Dogs
• Fluorescent Dyes (metabolism)
• Fura-2 (metabolism)
• Histamine (metabolism)
• Horseshoe Crabs (metabolism)
• Humans
• Mast Cells (drug effects, physiology)
• Mastocytoma
• Pertussis Toxin (pharmacology)
• Tumor Cells, Cultured