An alternatively spliced variant of cathepsin E in human gastric adenocarcinoma cells.

Abstract

Splice variant and authentic mRNAs for procathepsin E were measured at a ratio of 5:1 and 1:2 in Kato 3 and AGS cells, two human gastric adenocarcinoma cell lines. As a result of the precise splicing of the 3'-end of exon 6 to the 5'-end of exon 8, the variant lacked the 142 bp of exon 7 which encodes the second of the Asp residues that operate the catalytic mechanism of aspartic proteinases.

• Authors: Peter J Tatnell, Matthew Cook, John Kay
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 1625 Issue 2 Pg. 203-6 (Jan 27 2003) ISSN: 0006-3002 [Print] Netherlands
• PMID: 12531480 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Enzyme Precursors
• RNA, Messenger
• Cathepsins
• procathepsin E
• Cathepsin E

Topics

• Adenocarcinoma (metabolism)
• Alternative Splicing
• Cathepsin E (genetics, metabolism)
• Cathepsins (genetics, metabolism)
• Enzyme Precursors (genetics, metabolism)
• Exons
• Genetic Variation
• Humans
• Introns
• Polymerase Chain Reaction
• RNA, Messenger (analysis, isolation & purification)
• Stomach Neoplasms (metabolism)
• Tumor Cells, Cultured