Mechanism of cytotoxicity of turbinamide in vitro.

Abstract

BACKGROUND:

Previously, we demonstrated that turbinamide isolated from the marine ascidia Sydnium Turbinatum, exerts a selective cytotoxicity on glioma cells. Now we have investigated the mechanism of its cytotoxic effect on two different cell lines: C6 rat glioma cells and J774 murine monocyte/macrophages.

MATERIALS AND METHODS:

Cell viability, membrane lipoperoxidation, DNA fragmentation and apoptosis were studied in C6 and J774 cells incubated with turbinamide for 24 hours.

RESULTS:

Turbinamide (0.01-100 micrograms/ml) induced a dose-dependent inhibition of C6 cell viability (88.0 +/- 3.0%; 80.0 +/- 1.2%; 38.0 +/- 5.0%; 23.6 +/- 1.3% and 9.1 +/- 2.3%) with respect to the control (100% viability). Moreover, turbinamide (0.01-100 micrograms/ml) increased lipoperoxidation of C6 cells (1.21 +/- 0.11; 9.03 +/- 1.6; 17.8 +/- 1.6 and 45.03 +/- 1.01 ngMDA/1 x 10(6) cells) with respect to unstimulated cells (0.6 +/- 0.1 ngMDA/1 x 10(6) cells) that was accompanied by DNA damage, having no effect on J774. Interestingly, turbinamide (0.1-100 micrograms/ml) induced apoptosis in C6 cells.

CONCLUSIONS:

Our study showed that the selective cytotoxicity of turbinamide to C6 cells is due to apoptosis, suggesting that turbinamide may be useful in the therapy of glioma.

• Authors: Giuseppe Esposito, Anna Aiello, Sabina Carbonelli, Marialuisa Menna, Ernesto Fattorusso, Teresa Iuvone
• Journal: Anticancer research (Anticancer Res) 2002 Sep-Oct Vol. 22 Issue 5 Pg. 2827-31 ISSN: 0250-7005 [Print] Greece
• PMID: 12530004 (Publication Type: Journal Article)

Chemical References

• Alkanes
• Amides
• Antineoplastic Agents
• DNA, Neoplasm
• turbinamide
• DNA

Topics

• Alkanes (pharmacology, toxicity)
• Amides (pharmacology, toxicity)
• Animals
• Antineoplastic Agents (pharmacology, toxicity)
• Apoptosis (drug effects)
• Cell Line
• Cell Membrane (drug effects, metabolism)
• Cell Survival (drug effects)
• DNA (metabolism)
• DNA, Neoplasm (metabolism)
• Glioma (drug therapy, metabolism, pathology)
• Lipid Peroxidation (drug effects)
• Macrophages (cytology, drug effects, metabolism)
• Mice
• Rats
• Tumor Cells, Cultured