Abstract | PURPOSE: METHODS:
FITC-labeled decoy was injected into the subretinal space of rat eyes by the HVJ- liposome delivery system, and 3 days later, choroidal neovascularization was induced by laser photocoagulation. The eyes were removed and the transfected cells were detected by fluorescence microscopy and also detected by immunohistochemistry. The degree of neovascularization was evaluated by fluorescein angiography. RESULTS: The decoy was transfected into the retinal pigment epithelial (RPE) cells, inner and outer segment of the photoreceptors at 3 days after the injection. When choroidal neovascularization was induced, highly effective transfection of the decoy was observed 3 to 14 days after photocoagulation, after which the level decreased. Decoys were transfected into the RPE cells and macrophages in the choroidal neovascularization. The eyes transfected with NF-kappaB decoy showed a weaker leakage in fluorescein angiograms than that of the control eyes transfected with scrambled decoy. CONCLUSIONS:
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Authors | Nobuo Jo, Nahoko Ogata, Motokuni Aoki, Tsuyoshi Otsuji, Ryuichi Morishita, Yasufumi Kaneda, Miyo Matsumura |
Journal | Current eye research
(Curr Eye Res)
Vol. 24
Issue 6
Pg. 465-73
(Jun 2002)
ISSN: 0271-3683 [Print] England |
PMID | 12525974
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Fluorescent Dyes
- Liposomes
- NF-kappa B
- Fluorescein-5-isothiocyanate
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Topics |
- Animals
- Binding Sites
(genetics)
- Choroidal Neovascularization
(pathology, therapy)
- Fluorescein Angiography
- Fluorescein-5-isothiocyanate
- Fluorescent Dyes
- Genetic Therapy
- Immunohistochemistry
- Liposomes
- NF-kappa B
(metabolism)
- Rats
- Rats, Inbred BN
- Stereoisomerism
- Transfection
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