Immunoactivation of lectin-conjugated praecoxin A on IL-6, IL-12 expression.

Abstract

Lectin-conjugated praecoxin A is a compound, which is combined Wheat Germ Agglutinin (WGA) Lectin with praecoxin A and also known to have an anti-tumor activity. In our lab, in order to investigate its immune reaction other than the anti-tumor activity ever known, we examined cytokines such as IL-6 and IL-12 through their mRNA expressions, which are generally secreted by macrophage both in vivo and in vitro. To analyze, we used RT-PCR for total RNAs of macrophages. As a result, we obtained that both in vitro and in vivo, lectin-conjugated praecoxin A showed an interesting increase on IL-6 and IL-12 even though it may be little hard to say the conjugated form is absolutely more effective than that of lectin or praecoxin A alone for immune response activities. Those results suggest that the conjugated form may give an additional opportunity in a future therapeutic use over its immuno activation properties.

• Authors: Seong-Soo Joo, Jae-Kwon Chang, Jeong-Hwan Park, Hee-Chul Kang, Do-Ik Lee
• Journal: Archives of pharmacal research (Arch Pharm Res) Vol. 25 Issue 6 Pg. 954-63 (Dec 2002) ISSN: 0253-6269 [Print] Korea (South)
• PMID: 12510853 (Publication Type: Journal Article)

Chemical References

• Interleukin-6
• Plant Lectins
• RNA, Messenger
• Tannins
• Wheat Germ Agglutinins
• Interleukin-12

Topics

• Animals
• Cells, Cultured
• Interleukin-12 (biosynthesis, genetics)
• Interleukin-6 (biosynthesis, genetics)
• Macrophage Activation (drug effects, immunology)
• Macrophages, Peritoneal (drug effects, immunology, metabolism)
• Male
• Mice
• Mice, Inbred ICR
• Plant Lectins (immunology, metabolism, pharmacology)
• RNA, Messenger (biosynthesis)
• Tannins (immunology, metabolism, pharmacology)
• Wheat Germ Agglutinins (immunology, metabolism, pharmacology)