HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

[Effects of retinoid Ro 40-8757 on human cancer cell lines in vitro].

AbstractBACKGROUND & OBJECTIVES:
The retinoids are potent anti-tumor drugs affecting cellular proliferation and inducing cell differetiation or apoptosis. This study was designed to investigate the anti-proliferative effect of a new-developed retinoid, Ro 40-8757, on cell structure, cell cycle and cell cycle proteins of four human cancer cell lines in vitro in order to reveal its probable mechanism.
METHODS:
MTT assay was used to determine the anti-proliferative effects of Ro 40-8757 on human cancer cell lines CCL-187, CCL-229, JF-305, and ASPC-1. Microscopy was used to observe CCL-187 morphological changes. Flow cytometry was performed to investigate the influence of Ro 40-8757 on cell cycle. Western blot analysis was conducted to detect the cell cycle proteins p16, p21, and p27 as to discuss the possible mechanism.
RESULTS:
Ro 40-8757 significantly inhibited the growth of the four cancer cell lines in a dose-, time-dependent manner and without any signs of cytotoxicity, differentiation, or apoptosis. After treating with Ro 40-8757, the cell of CCL-187 was arrested at G0/G1 phase. Both p21 and p27 were rapidly increasing at the first 12 hours after exposed to the agent, then decreasing slowly in the 24, 48 hours, and increasing again in 72 to 144 hours. P16 did not express at all either before or after agent treatment.
CONCLUSION:
The results suggest that Ro 40-8757 inhibits the growth of human cancer cell lines in vitro by means of cell cycle arrest (mediated by up-regulating cell cycle protein P21 and P27) instead of cytotoxic effects, differentiation, or apoptosis.
AuthorsJing Lu, Jin-dan Song
JournalAi zheng = Aizheng = Chinese journal of cancer (Ai Zheng) Vol. 21 Issue 10 Pg. 1051-6 (Oct 2002) China
PMID12508641 (Publication Type: English Abstract, Journal Article)
Chemical References
  • Antineoplastic Agents
  • CDKN1A protein, human
  • Cell Cycle Proteins
  • Cyclin-Dependent Kinase Inhibitor p21
  • Cyclins
  • Morpholines
  • Retinoids
  • Tumor Suppressor Proteins
  • Cyclin-Dependent Kinase Inhibitor p27
  • mofarotene
Topics
  • Antineoplastic Agents (pharmacology)
  • Blotting, Western
  • Cell Cycle (drug effects)
  • Cell Cycle Proteins (drug effects, metabolism)
  • Cell Division (drug effects)
  • Cell Survival (drug effects)
  • Cyclin-Dependent Kinase Inhibitor p21
  • Cyclin-Dependent Kinase Inhibitor p27
  • Cyclins (drug effects, metabolism)
  • Dose-Response Relationship, Drug
  • Humans
  • Inhibitory Concentration 50
  • Microscopy, Electron
  • Microscopy, Electron, Scanning
  • Morpholines (pharmacology)
  • Retinoids (pharmacology)
  • Tumor Cells, Cultured (drug effects, metabolism, ultrastructure)
  • Tumor Suppressor Proteins (drug effects, metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: