Simple and sensitive determination of the new antitumor drug CKD-602 in human plasma by liquid chromatography.

Abstract

A simple and sensitive high-performance liquid chromatographic with fluorescence detection method has been developed and validated for the determination of the new antitumor agent CKD-602 in human plasma. Plasma proteins were precipitated with methanol and the samples were acidified with 7% (v/v) perchloric acid. The supernatants were analyzed by HPLC using a Capcell Pak C(18) UG120 column and a mixture of methanol-0.1 M hexane-1-sulfonic acid in methanol-0.01 M TEMED in water at pH 6.0 (40:1:59, v/v) as the mobile phase. The lower limit of quantification was 0.2 ng/ml using 200 microliter plasma samples. Mean within-run precision and between-run precision at six tested concentrations (0.2-400 ng/ml) were </=10% and mean accuracy was </=15%. Stability studies showed that CKD-602 is stable in both plasma and methanol extracts for at least 3 months at -30 degrees C. The described method was used for the pharmacokinetic analysis of CKD-602 during clinical phase I studies, in patients with solid tumors.

• Authors: Joo Youn Cho, Hyo Bum Seo, Kyung Sang Yu, Kyun Seop Bae, So Young Yi, In Jin Jang, Sang Goo Shin
• Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (J Chromatogr B Analyt Technol Biomed Life Sci) Vol. 784 Issue 1 Pg. 25-31 (Jan 25 2003) ISSN: 1570-0232 [Print] Netherlands
• PMID: 12504179 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• belotecan
• Camptothecin

Topics

• Antineoplastic Agents (blood)
• Camptothecin (analogs & derivatives, blood)
• Chromatography, High Pressure Liquid (methods)
• Humans
• Reproducibility of Results
• Sensitivity and Specificity