Abstract |
Recruitment of macrophages plays an important role in initiation of atheroma, but their involvement in cholesterol clearance during regression is unknown. We developed a mouse model to quantitate cholesterol clearance from a depot of cationized LDL injected into a leg muscle, which evokes a sterile inflammatory reaction. In the CCR2(-/-) mice, cholesterol clearance was significantly slower than in C57BL controls because of decrease in cholesteryl ester (CE) hydrolysis, which is mandatory prior to cholesterol efflux. In CCR2(-/-) mice, macrophage recruitment to the injected site, identified by immunohistochemistry, was markedly delayed. CE hydrolysis was also significantly reduced in thioglycollate elicited peritoneal exudate cells of CCR2(-/-) mice, related to paucity of macrophages in the cell differential. The present study provides definite evidence that recruitment of macrophages is required for LDL cholesterol clearance, which plays a prominent role in regression of an atheroma.
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Authors | Olga Stein, Yedida Dabach, Mazal Ben-Naim, Gideon Halperin, Israel F Charo, Yechezkiel Stein |
Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 300
Issue 2
Pg. 477-81
(Jan 10 2003)
ISSN: 0006-291X [Print] United States |
PMID | 12504109
(Publication Type: Journal Article)
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Chemical References |
- Ccr2 protein, mouse
- Cholesterol Esters
- Cholesterol, LDL
- Lipids
- Receptors, CCR2
- Receptors, Chemokine
- Cholesterol
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Topics |
- Animals
- Biological Transport
- Cell Movement
- Cells, Cultured
- Cholesterol
(metabolism)
- Cholesterol Esters
(metabolism)
- Cholesterol, LDL
(metabolism)
- Female
- Kinetics
- Lipids
(blood)
- Macrophages
(physiology)
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Monocytes
(physiology)
- Muscle, Skeletal
(cytology, metabolism)
- Receptors, CCR2
- Receptors, Chemokine
(genetics, physiology)
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