Abstract |
Desmoplastic small round cell tumor (DSRCT) is defined genetically by the chimeric fusion of the Ewing's sarcoma and Wilms' tumor genes, generating a novel transcription factor, EWS-WT1. By using cells with inducible EWS-WT1 to screen high-density microarrays, we identified BAIAP3 as a transcriptional target of the chimera. The BAIAP3 promoter is specifically bound in vivo by the (-KTS) isoform of EWS-WT1, consistent with its activation in reporter assays. BAIAP3 encodes a protein implicated in regulated exocytosis, which is colocalized with a secreted growth factor within cytoplasmic organelles. Ectopic expression of BAIAP3 in tumor cells dramatically enhances growth in low serum and colony formation in soft agar. BAIAP3 therefore encodes a transcriptional target of an oncogenic fusion protein that implicates the regulated exocytotic pathway in cancer cell proliferation.
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Authors | Rachel E Palmer, Sean Bong Lee, Jenise C Wong, Paul A Reynolds, Hong Zhang, Vivi Truong, Jonathan D Oliner, William L Gerald, Daniel A Haber |
Journal | Cancer cell
(Cancer Cell)
Vol. 2
Issue 6
Pg. 497-505
(Dec 2002)
ISSN: 1535-6108 [Print] United States |
PMID | 12498718
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Angiogenesis Inhibitors
- Oncogene Proteins, Fusion
- Proteins
- RNA-Binding Protein EWS
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Topics |
- Angiogenesis Inhibitors
- Animals
- Base Sequence
- Blotting, Northern
- Carcinoma, Small Cell
(genetics, metabolism)
- Cell Transformation, Neoplastic
(genetics)
- Exocytosis
(physiology)
- Gene Expression Regulation, Neoplastic
- Genes, Wilms Tumor
- Humans
- In Situ Hybridization
- Molecular Sequence Data
- Mosaicism
- Oncogene Proteins, Fusion
(genetics)
- Osteosarcoma
(genetics, metabolism)
- Promoter Regions, Genetic
- Proteins
(genetics, metabolism)
- RNA-Binding Protein EWS
(genetics)
- Transcription, Genetic
- Tumor Cells, Cultured
(ultrastructure)
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