Abstract | BACKGROUND: METHODS: Cell proliferation was measured by MTT assay. The cytotoxic effects were determined by lactate dehydrogenase measurements. The intracellular calcium concentration ([Ca(2+)](i)) was measured by a dual-wavelength spectrometer system. TUNEL assay and flow cytometry were performed to measure percentage of apoptotic cells. A colorimetric assay was to measure caspases activities. RESULTS: CONCLUSIONS:
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Authors | Jiun-Yih Yeh, William J Huang, Shu-Fen Kan, Paulus S Wang |
Journal | The Prostate
(Prostate)
Vol. 54
Issue 2
Pg. 112-24
(Feb 01 2003)
ISSN: 0270-4137 [Print] United States |
PMID | 12497584
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2002 Wiley-Liss, Inc. |
Chemical References |
- Antineoplastic Agents
- Bufanolides
- Materia Medica
- Sodium-Potassium-Exchanging ATPase
- Calcium
- cinobufagin
- bufalin
|
Topics |
- Antineoplastic Agents
(pharmacology)
- Apoptosis
- Bufanolides
(pharmacology)
- Calcium
(metabolism)
- Cell Division
(drug effects)
- Dose-Response Relationship, Drug
- Humans
- Intracellular Fluid
(chemistry)
- Male
- Materia Medica
(pharmacology)
- Prostatic Neoplasms
(pathology)
- Sodium-Potassium-Exchanging ATPase
(antagonists & inhibitors)
- Tumor Cells, Cultured
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