Abstract |
Prodelphinidin B-2 3-O-gallate, a proanthocyanidin gallate isolated from green tea leaf, was investigated for its anti-herpes simplex virus type 2 properties in vitro. Prodelphinidin B-2 3'-O-gallate exhibited antiviral activity with IC50 of 5.0 +/-1.0 microM and 1.6 +/-0.3 pM for XTT and plaque reduction (PRA) assays, respectively. Cytotoxicity assay had shown that prodelphinidin B-2 3'-O-gallate possessed cytotoxic effect toward Vero cell at concentration higher than its IC50. The 50% cytotoxic concentration for cell growth (CC50) was 33.3 +/- 3.7 microM. Thus, the selectivity index (SI) (ratio of IC50 to CC50) for XTT assay and PRA was 6.7 and 20.8, respectively. Prodelphinidin B-2 3'-O-gallate significantly reduced viral infectivity at concentrations 10 microM or more. Result of time-of-addition studies suggested that prodelphinidin B-2 3'-O-gallate affected the late stage of HSV-2 infection. In addition, it was also shown to inhibit the virus from attaching and penetrating into the cell. Thus, prodelphinidin B-2 3'-O-gallate was concluded to possess antiviral activity with mechanism of inhibiting viral attachment and penetration, and disturbing the late stage of viral infection.
|
Authors | Hua-Yew Cheng, Chun-Ching Lin, Ta-Chen Lin |
Journal | Antiviral chemistry & chemotherapy
(Antivir Chem Chemother)
Vol. 13
Issue 4
Pg. 223-9
(Jul 2002)
ISSN: 0956-3202 [Print] England |
PMID | 12495210
(Publication Type: Journal Article)
|
Chemical References |
- Anthocyanins
- Antiviral Agents
- Proanthocyanidins
- Tea
- prodelphinidin B-2 3'-O-gallate
- proanthocyanidin
|
Topics |
- Animals
- Anthocyanins
(chemistry, pharmacology)
- Antiviral Agents
(chemistry, pharmacology)
- Chlorocebus aethiops
- Dose-Response Relationship, Drug
- Herpesvirus 2, Human
(drug effects, physiology)
- Inhibitory Concentration 50
- Plant Leaves
(chemistry)
- Proanthocyanidins
- Tea
(chemistry)
- Time Factors
- Vero Cells
(drug effects, virology)
- Viral Plaque Assay
|