Fructose-1,6-diphosphate alone and in combination with cyclosporine potentiates rat cardiac allograft survival and inhibits lymphocyte proliferation and interleukin-2 expression.

Abstract

BACKGROUND:

Fructose-1,6-diphosphate (FDP) reduces postischemic reperfusion injury and is used alone and in combination with cyclosporine A (CsA) as an immunosuppressant.

METHODS:

Wistar-Furth rat hearts were grafted to Lewis rats. Activated T-cell proliferation, viability, and interleukin-2 expression were determined.

RESULTS:

Mean survival in days were: saline 7.12+/-0.64, FDP 350 mg/kg perioperatively 13.5+/-1.4, FDP 350 mg/kg twice daily 11.4+/-0.75, CsA 2.5 mg/kg daily 12+/-0.81, CsA 5.0 mg/kg daily 12.4+/-0.81, CsA 2.5 mg/kg + FDP 350 mg/kg twice daily 17.6+/-0.4, and CsA 5 mg/kg + FDP 350 mg/kg twice daily 28.2+/-0.97. FDP maximally inhibits T-cell proliferation and concomitantly increases cell viability at 5,000 to 500 microg/mL, whereas CsA inhibits at 500 ng/mL. FDP completely inhibited interleukin-2 expression at 5,000 to 500 microg/mL, whereas CsA partially inhibited at 50 to 500 ng/mL.

CONCLUSION:

FDP + CsA prolongs cardiac survival and FDP inhibits T-cell proliferation.

• Authors: Angel K Markov, Thomas S Rayburn, David S Talton, Donald E Netherland, Charles Moore, Bobby Heath, Hari H Cohly
• Journal: Transplantation (Transplantation) Vol. 74 Issue 11 Pg. 1651-4 (Dec 15 2002) ISSN: 0041-1337 [Print] United States
• PMID: 12490806 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Cardiovascular Agents
• Fructosediphosphates
• Immunosuppressive Agents
• Interleukin-2
• Cyclosporine
• fructose-1,6-diphosphate

Topics

• Animals
• Cardiovascular Agents (therapeutic use)
• Cell Division (drug effects)
• Cyclosporine (therapeutic use)
• Drug Therapy, Combination
• Fructosediphosphates (therapeutic use)
• Graft Survival (drug effects)
• Heart Transplantation
• Immunosuppressive Agents (therapeutic use)
• Interleukin-2 (antagonists & inhibitors)
• Rats
• Rats, Inbred Lew
• Rats, Inbred WF
• Rats, Sprague-Dawley
• T-Lymphocytes (pathology)
• Transplantation, Heterotopic