Characterization of human alpha-dystrobrevin isoforms in HL-60 human promyelocytic leukemia cells undergoing granulocytic differentiation.

Abstract	The biochemical properties and spatial localization of the protein alpha-dystrobrevin and other isoforms were investigated in cells of the human promyelocytic leukemia line HL-60 granulocytic differentiation as induced by retinoic acid (RA). Alpha-dystrobrevin was detected both in the cytosol and the nuclei of these cells, and a short isoform (gamma-dystrobrevin) was modified by tyrosine phosphorylation soon after the onset of the RA-triggered differentiation. Varying patterns of distribution of alpha-dystrobrevin and its isoforms could be discerned in HL-60 promyelocytes, RA-differentiated mature granulocytes, and human neutrophils. Moreover, the gamma-dystrobrevin isoform was found in association with actin and myosin light chain. The results provide new information about potential involvement of alpha-dystrobrevin and its splice isoforms in signal transduction in myeloid cells during induction of granulocytic differentiation and/or at the commitment stage of differentiation or phagocytic cells.
Authors	Agné Kulyte, Ruta Navakauskiene, Grazina Treigyte, Arunas Gineitis, Tomas Bergman, Karl-Eric Magnusson
Journal	Molecular biology of the cell (Mol Biol Cell) Vol. 13 Issue 12 Pg. 4195-205 (Dec 2002) ISSN: 1059-1524 [Print] United States
PMID	12475945 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Actins Cytoskeletal Proteins Dystrophin-Associated Proteins Membrane Proteins Myosin Light Chains Protein Isoforms dystrobrevin Tyrosine Trypsin
Topics	Actins (metabolism) Cell Differentiation Cell Nucleus (metabolism) Cytoskeletal Proteins (chemistry, metabolism) Cytosol (metabolism) Dystrophin-Associated Proteins Electrophoresis, Polyacrylamide Gel Granulocytes (cytology, metabolism) HL-60 Cells Humans Immunoblotting Membrane Proteins (chemistry, metabolism) Microscopy, Confocal Microscopy, Fluorescence Myosin Light Chains (metabolism) Neutrophils (metabolism) Phagocytosis Phosphorylation Precipitin Tests Protein Isoforms Signal Transduction Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Time Factors Trypsin (pharmacology) Tyrosine (metabolism)

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