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DNA vaccination against hepatitis E virus infection in cynomolgus macaques.

AbstractBACKGROUND:
: The feasibility of DNA vaccination against hepatitis E in non-human primates has not been evaluated. In the present study a full-length hepatitis E virus (HEV) open reading frame (ORF)2 (Burmese strain) was assembled, cloned, and used for genetic immunization of cynomolgus macaques (cynos), which were subsequently challenged with a heterologous HEV strain (Mexico).
METHODS:
: Four cynos were vaccinated intramuscularly with the HEV ORF2 DNA cassette and one animal was vaccinated with a mock DNA construct.
RESULTS:
: Following vaccination anti-HEV antibodies were detected in the four HEV-DNA-vaccinated cynos, but not in the control animal. When challenged, two of the four HEV-DNA-vaccinated cynos were protected against HEV infection and had no elevated alanine aminotransferase activity, viremia, or fecal shedding. The two other DNA-vaccinated animals developed HEV infection and disease.
CONCLUSION:
: These findings demonstrate the feasibility of DNA vaccination for the protection of HEV infection and warrant further studies to explore routes other than intramuscular for induction of a stronger and efficacious immune response.
AuthorsSaleem Kamili, John Spelbring, Krzysztof Krawczynski
JournalJournal of gastroenterology and hepatology (J Gastroenterol Hepatol) Vol. 17 Suppl 3 Pg. S365-9 (Dec 2002) ISSN: 1440-1746 [Electronic] Australia
PMID12472965 (Publication Type: Journal Article)
CopyrightCopyright 2002 Blackwell Publishing Asia Pty Ltd
Chemical References
  • Antibodies, Viral
  • ORF2 protein, Hepatitis E virus
  • Vaccines, DNA
  • Viral Hepatitis Vaccines
  • Viral Proteins
Topics
  • Animals
  • Antibodies, Viral (blood)
  • Hepatitis E (prevention & control)
  • Hepatitis E virus (immunology)
  • Macaca fascicularis
  • Vaccines, DNA
  • Viral Hepatitis Vaccines
  • Viral Load
  • Viral Proteins

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