Effects of gentamicin, lipopolysaccharide, and contrast media on immortalized proximal tubular cells.

Abstract

Aminoglycosides are widely used in the treatment of gram-negative bacterial infections. Gentamicin (GE) acts mainly in proximal tubular cells, where it is uptake via organic anion transport system and it induces a high incidence of nephrotoxicity, which is characterized by tubular necrosis [5] leading to acute renal failure in 10 to 50% of patients. Gram-negative bacteria has lipopolysaccharide (LPS) which is an endotoxin that cause renal damage. [1] Moreover, many patients are undergone exams using radiologic contrast, which is a risk factor to induce a hemodynamic change in the kidney and to develop acute renal failure. [6] Intracellular calcium [Ca2+]i is involved in renal cellular injury [7,3] and maybe mediate the effects provoked by these drugs. This study was performed to evaluate necrosis, apoptosis, and intracellular calcium levels ([Ca2+]i) in LLC-PK1 (epithelial cell line from pig kidney) induced by GE associated with LPS and a low-osmolality media, Hexabrix (HE).

• Authors: Maria A Cunha, Nestor Schor
• Journal: Renal failure (Ren Fail) Vol. 24 Issue 6 Pg. 687-90 (Nov 2002) ISSN: 0886-022X [Print] England
• PMID: 12472192 (Publication Type: Journal Article)

Chemical References

• Anti-Bacterial Agents
• Contrast Media
• Gentamicins
• Lipopolysaccharides
• Calcium
• Ioxaglic Acid

Topics

• Animals
• Anti-Bacterial Agents (adverse effects, pharmacology)
• Apoptosis (drug effects, physiology)
• Calcium (analysis)
• Contrast Media (adverse effects, pharmacology)
• Disease Models, Animal
• Gentamicins (adverse effects, pharmacology)
• In Vitro Techniques
• Intracellular Fluid (chemistry, drug effects)
• Ioxaglic Acid (adverse effects, pharmacology)
• Kidney Diseases (chemically induced, pathology, physiopathology)
• Kidney Tubules, Proximal (drug effects, pathology, physiopathology)
• LLC-PK1 Cells (drug effects, pathology, physiology)
• Lipopolysaccharides (adverse effects, pharmacology)
• Necrosis
• Swine
• Time Factors