Huperzine B protects rat pheochromocytoma cells against oxygen-glucose deprivation-induced injury.

Abstract

AIM:

To test the ability of huperzine B (HupB) to alleviate injury from oxygen-glucose deprivation (OGD) in the rat pheochromocytoma line PC12 cells.

METHODS:

After OGD for 3 h and reoxygenation for 24 h, neuronal morphology was observed by phase-contrast microscopy; cell survival was quantified by the reduction of MTT; malondialdehyde (MDA) was determined by the thiobarbituric acid; superoxide dismutase (SOD) was assayed by a modification of the xanthine/xanthine oxidase; and lactate (LA) was measured according to Marbach and Weil.

RESULTS:

OGD for 3 h and reoxygenation for 24 h triggered death in nearly 70 % of cells, along with major changes in morphology and biochemistry including elevated level of MDA, SOD activity, and LA content. Cells pretreated with HupB 1-100 micromol/L for 2 h showed significantly improved survival and reduced biochemical and morphologic signs of toxicity.

CONCLUSION:

HupB protected PC12 cells against OGD-induced injury, most likely by alleviating disturbances of oxidative and energy metabolism.

• Authors: Zhi-Fei Wang, Jin Zhou, Xi-Can Tang
• Journal: Acta pharmacologica Sinica (Acta Pharmacol Sin) Vol. 23 Issue 12 Pg. 1193-8 (Dec 2002) ISSN: 1671-4083 [Print] United States
• PMID: 12466060 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Alkaloids
• Neuroprotective Agents
• huperzine B
• Lactic Acid
• Malondialdehyde
• Superoxide Dismutase
• Glucose

Topics

• Alkaloids (isolation & purification, pharmacology)
• Animals
• Cell Hypoxia
• Cell Survival (drug effects)
• Glucose (deficiency)
• Huperzia (chemistry)
• Lactic Acid (metabolism)
• Malondialdehyde (metabolism)
• Neuroprotective Agents (isolation & purification, pharmacology)
• PC12 Cells
• Pheochromocytoma (pathology)
• Plants, Medicinal (chemistry)
• Rats
• Superoxide Dismutase (metabolism)