Abstract |
Cyclooxygenase-2 (COX-2), the inducible COX isozyme, plays a key role in intestinal tumorigenesis. We have demonstrated recently that COX-2 protein is induced in the polyp stroma near the intestinal luminal surface in the Apc(Delta716) mouse, a model for human familial adenomatous polyposis, and stimulate tumor angiogenesis. However, the precise cell types that express COX-2 are still to be determined. By immunohistochemical analysis, here we show that the majority of COX-2-expressing cells in the intestinal polyps of Apc(Delta716) mice are fibroblasts and endothelial cells. Furthermore, the COX-2-expressing cells in human familial adenomatous polyposis polyps are also fibroblasts and endothelial cells. In contrast, bone marrow-derived cells such as macrophages and leukocytes express little COX-2 protein in the intestinal polyps. These results clearly indicate that fibroblasts and endothelial cells play important roles in polyp expansion by expressing COX-2, resulting in tumor angiogenesis.
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Authors | Masahiro Sonoshita, Kazuaki Takaku, Masanobu Oshima, Ken-ichi Sugihara, Makoto M Taketo |
Journal | Cancer research
(Cancer Res)
Vol. 62
Issue 23
Pg. 6846-9
(Dec 01 2002)
ISSN: 0008-5472 [Print] United States |
PMID | 12460897
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Actins
- Antigens, CD34
- Antigens, Differentiation
- Isoenzymes
- Membrane Proteins
- Vimentin
- monocyte-macrophage differentiation antigen
- Cyclooxygenase 2
- PTGS2 protein, human
- Prostaglandin-Endoperoxide Synthases
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Topics |
- Actins
(biosynthesis)
- Adenomatous Polyposis Coli
(enzymology, metabolism, pathology)
- Animals
- Antigens, CD34
(biosynthesis)
- Antigens, Differentiation
(biosynthesis)
- Cyclooxygenase 2
- Endothelium, Vascular
(cytology, enzymology, metabolism)
- Fibroblasts
(enzymology, metabolism)
- Humans
- Immunohistochemistry
- Intestinal Polyps
(enzymology, pathology)
- Isoenzymes
(biosynthesis)
- Macrophages
(enzymology, metabolism)
- Membrane Proteins
- Mice
- Mice, Knockout
- Prostaglandin-Endoperoxide Synthases
(biosynthesis)
- Stromal Cells
(enzymology, metabolism)
- Vimentin
(biosynthesis)
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