Abstract | BACKGROUND & OBJECTIVE: METHODS:
Cyclin G2 cDNA was synthesized by reverse transcription-polymerase chain reaction (RT-PCR) and inserted into the pIRESneo vector at BamH I and BstX I sites to generate the recombinant plasmid pIRES-G2. The pIRES-G2 and pIRESneo plasmids were then individually introduced into HeLa cancer cell line through lipofectamine mediated transfection. After two weeks' selection in culture medium containing G418, the number of colonies was counted and the transfectant cells were morphologically observed. RESULTS: The colony-forming efficiency of the cells transfected with pIRES-G2 construct was much lower compared with that with the control parental vector pIRESneo. The colony numbers were 76.7 +/- 24.8 and 18 +/- 10.4 in control and experimental groups, respectively, with a colony forming rate of 23.4% in the pIRES-G2 group. Furthermore, pIRES-G2 transfected cells showed a senescent morphology. CONCLUSION: Ectopic overexpression of cyclin G2 in cancer cell line HeLa could inhibit cell proliferation significantly.
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Authors | Yu-lou Tian, Fu-rong Liu, Jie Liu, Li Jiang, Yang Luo, Xue Zhang |
Journal | Ai zheng = Aizheng = Chinese journal of cancer
(Ai Zheng)
Vol. 21
Issue 6
Pg. 577-81
(Jun 2002)
China |
PMID | 12452053
(Publication Type: English Abstract, Journal Article)
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Chemical References |
- CCNG2 protein, human
- Cyclin G2
- Cyclins
- DNA, Complementary
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Topics |
- Cell Division
- Cloning, Molecular
- Cyclin G2
- Cyclins
(genetics)
- DNA, Complementary
- Gene Expression
- Genetic Vectors
- HeLa Cells
- Humans
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