Abstract | BACKGROUND: METHODS: RESULTS: Migration times of the isoforms were identical in both populations. Asialotransferrin was missing in teetotalers but present in 92% of alcohol abusers. Disialotransferrin was higher in those who consumed excessive amounts of alcohol, whereas mean trisialotransferrin concentration was not affected by alcohol abuse. At cutoffs to maximize sensitivity and specificity, these values were 0.92 and 1 [mean ROC area (MRa), 0.96; 95% confidence interval (CI), 0.93-0.99] for asialotransferrin; 0.84 and 0.94 for the sum of asialo- + disialotransferrin (MRa, 0.94; 95% CI, 0.91-0.98); 0.79 and 0.94 for disialotransferrin (MRa, 0.89; 95% CI, 0.84-0.94); 0.62 and 0.53 for trisialotransferrin (MRa, 0.58; 95% CI, 0.49-0.68); 0.79 and 0.82 for a 3% %CDT; and 0.83 and 0.69 for a 2.6% cutoff (MRa, 0.87; 95% CI, 0.81-0.92). Current markers lack sensitivity (<0.65). Transferrins were not significantly correlated with serum enzymes and mean erythrocyte volume. CONCLUSIONS: CZE-isolated desialylated transferrin isoforms allowed differentiation between chronic alcohol abusers and teetotalers.
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Authors | Franz J Legros, Vincent Nuyens, Eddy Minet, Philippe Emonts, Karim Zouaoui Boudjeltia, Anne Courbe, Jean-Luc Ruelle, Jacques Colicis, Francois de L'Escaille, Jean-Pol Henry |
Journal | Clinical chemistry
(Clin Chem)
Vol. 48
Issue 12
Pg. 2177-86
(Dec 2002)
ISSN: 0009-9147 [Print] England |
PMID | 12446474
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers
- Protein Isoforms
- Transferrin
- carbohydrate-deficient transferrin
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Topics |
- Adult
- Alcoholism
(diagnosis)
- Biomarkers
(blood)
- Chromatography, Ion Exchange
- Electrophoresis, Capillary
- Female
- Humans
- Male
- Nephelometry and Turbidimetry
- Protein Isoforms
- ROC Curve
- Transferrin
(analogs & derivatives, analysis)
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