Abstract | INTRODUCTION: MATERIALS AND METHODS: This study examined the effect of inosine on ischemia-reperfusion-induced gut barrier dysfunction and on the associated lung injury. Twenty-four male Sprague-Dawley rats were divided into three groups. Eight were subjected to 60 min of superior mesenteric artery occlusion followed by 4 h of reperfusion. Eight had 100 mg/kg inosine prior to ischemia-reperfusion and 8 had sham laparotomy with encircling but not occlusion of the superior mesenteric artery. RESULTS: Rats treated with inosine had significantly less gut barrier dysfunction. Rats subjected to SMAO sustained a substantial lung injury and this was attenuated by inosine treatment. Serum cytokine levels were also significantly lower. CONCLUSIONS: We conclude that inosine has a beneficial effect in modulating both gut barrier dysfunction and distant organ injury in response to gut ischemia-reperfusion.
|
Authors | J F Dowdall, D C Winter, D J Bouchier-Hayes |
Journal | The Journal of surgical research
(J Surg Res)
Vol. 108
Issue 1
Pg. 61-8
(Nov 2002)
ISSN: 0022-4804 [Print] United States |
PMID | 12443716
(Publication Type: Journal Article)
|
Chemical References |
- Dextrans
- HSP72 Heat-Shock Proteins
- Heat-Shock Proteins
- Interleukin-6
- Tumor Necrosis Factor-alpha
- fluorescein isothiocyanate dextran
- Inosine
- Peroxidase
- Fluorescein-5-isothiocyanate
|
Topics |
- Animals
- Dextrans
(pharmacokinetics)
- Disease Models, Animal
- Fluorescein-5-isothiocyanate
(analogs & derivatives, pharmacokinetics)
- HSP72 Heat-Shock Proteins
- Heat-Shock Proteins
(metabolism)
- Inosine
(pharmacology)
- Interleukin-6
(blood)
- Intestinal Absorption
(drug effects)
- Intestinal Mucosa
(metabolism)
- Intestines
(blood supply, pathology)
- Lung
(blood supply, enzymology, pathology)
- Lung Diseases
(drug therapy, pathology)
- Male
- Mesenteric Artery, Superior
- Microcirculation
- Peroxidase
(metabolism)
- Pulmonary Circulation
- Rats
- Rats, Sprague-Dawley
- Reperfusion Injury
(drug therapy, pathology)
- Tumor Necrosis Factor-alpha
(metabolism)
|