Abstract | AIM: To study the expression of early growth response gene-1 (Egr-1 gene) and Bcl-X/(L) protein and its relationship with the cell apoptosis in human esophageal carcinoma (EC) and precancerous lesions. METHODS: In situ hybridization(ISH), immunohistochemistry (IHC) and TUNEL method were used respectively to detect Egr-1mRNA, Egr-1 protein, apoptosis related- protein Bcl-X/(L) and cell apoptosis in situ from 66 cases of esophageal squamous cell carcinoma and their upper cut edge and paracancerous mucosa. RESULTS: Egr-1 gene in situ hybridization, Bcl-X/(L) immunohistochemistry positive products were located in the cytoplasm, while Egr-1 immunohistochemistry and TUNEL positive signal were located in the nuclei. The apoptosis index(AI) and the frequency of apoptosis occurrence were increased gradually from precancerous lesion to cancer (P<0.01) and the expression of Egr-1mRNA and Egr-1 protein in dysplasia was the highest among all specimens (P<0.01). The AI of Egr-1 positive cancer tissues was much higher than that of Egr-1 negative cancer tissues (P<0.01), while the AI of Bcl-X/(L) positive cancer tissues was much lower than that of Bcl-X/(L) negative cancer tissues (P<0.01). The AI and Egr-1 expression were not correlated with invasiveness and lymphatic metastasis in EC. CONCLUSION: Cell apoptosis was present through esophageal carcinogenesis. The expression of Egr-1 mRNA and Egr-1 protein were high in precancerous lesion of esophagus. The AI was increased significantly in Egr-1 positive squamous cell carcinoma. Egr-1 might promote apoptotic effect. Egr-1 expression and cell apoptosis may have an important biological significance in esophageal carcinogenesis.
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Authors | Ming-Yao Wu, Ying-Rui Liang, Xian-Ying Wu, Chu-Xiang Zhuang |
Journal | World journal of gastroenterology
(World J Gastroenterol)
Vol. 8
Issue 6
Pg. 971-5
(Dec 2002)
ISSN: 1007-9327 [Print] United States |
PMID | 12439908
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- BCL2L1 protein, human
- DNA-Binding Proteins
- EGR1 protein, human
- Early Growth Response Protein 1
- Immediate-Early Proteins
- Proto-Oncogene Proteins c-bcl-2
- RNA, Messenger
- RNA, Neoplasm
- Transcription Factors
- bcl-X Protein
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Topics |
- Apoptosis
(genetics)
- Carcinoma, Squamous Cell
(genetics, metabolism, pathology)
- DNA-Binding Proteins
(genetics, metabolism)
- Early Growth Response Protein 1
- Esophageal Neoplasms
(genetics, metabolism, pathology)
- Gene Expression
- Humans
- Immediate-Early Proteins
- Immunohistochemistry
- In Situ Hybridization
- In Situ Nick-End Labeling
- Precancerous Conditions
(genetics, metabolism, pathology)
- Proto-Oncogene Proteins c-bcl-2
(metabolism)
- RNA, Messenger
(genetics, metabolism)
- RNA, Neoplasm
(genetics, metabolism)
- Transcription Factors
(genetics, metabolism)
- bcl-X Protein
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